Alotaibi Modhi O, Sonbol Hana S, Alwakeel Suaad S, Suliman Rasha S, Fodah Ramy A, Abu Jaffal Ahmad S, AlOthman Nouf I, Mohammed Afrah E
Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, 84428 Riyadh, Saudi Arabia.
Pharmaceutical Sciences Department, College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia.
Saudi J Biol Sci. 2020 Oct;27(10):2778-2789. doi: 10.1016/j.sjbs.2020.06.038. Epub 2020 Jun 27.
Several studies isolated fungal and bacterial species from extreme environments, such as Sabkha and hot deserts, as their natural habitat, some of which are of medicinal importance. Current research aimed investigating the microbial (fungi and bacteria) diversity and abundance in Sabkha and desert areas in Saudi Arabia. Soil samples from nine different geographical areas (Al-Aushazia lake, AlQasab, AlKasar, Tabuk, Al-Kharj, Al-Madina, Jubail, Taif and Abqaiq) were collected and cultured for microbial isolation. Isolated fungi and bacteria were identified by molecular techniques (PCR and sequencing). Based on 18S rDNA sequencing, 203 fungal species belonging to 33 genera were identified. The most common fungal genera were , , , and , while the most common species were and . By 16S rDNA sequencing 22 bacterial species belonging to only two genera, and , were identified. The most commonly isolated bacterial species were and . Some fungal species were confined to specific locations, such as and spp that were only isolated from Al-Aushazia soil. AlQasab soil had the highest microbial diversity among other areas with abundances of 23.5% and 4.4% of total fungi, and bacteria, respectively. Findings of this study show a higher degree of fungal diversity than that of bacteria in all studied areas. Further studies needed to investigate the connection between some isolated species and their habitat ecology, as well as to identify those of medicinal importance.
多项研究从诸如盐沼和炎热沙漠等极端环境中分离出真菌和细菌物种，将其作为自然栖息地，其中一些具有药用价值。当前的研究旨在调查沙特阿拉伯盐沼和沙漠地区的微生物（真菌和细菌）多样性及丰度。采集了来自九个不同地理区域（奥沙扎亚湖、卡斯卜、卡萨尔、塔布克、哈吉尔、麦地那、朱拜勒、塔伊夫和阿卜盖格）的土壤样本并进行培养以分离微生物。通过分子技术（聚合酶链式反应和测序）对分离出的真菌和细菌进行鉴定。基于18S核糖体DNA测序，鉴定出属于33个属的203种真菌。最常见的真菌属为、、、和，而最常见的物种为和。通过16S核糖体DNA测序，鉴定出仅属于两个属（和）的22种细菌。最常分离出的细菌物种为和。一些真菌物种局限于特定地点，例如和仅从奥沙扎亚土壤中分离得到。在其他地区中，卡斯卜土壤的微生物多样性最高，真菌和细菌的丰度分别占总真菌和细菌的23.5%和4.4%。本研究结果表明，在所有研究区域中真菌多样性程度高于细菌。需要进一步研究以调查一些分离出的物种与其栖息地生态之间的联系，以及鉴定出具有药用价值的物种。
