Chang Yujie, Sun Feifei, Sun Suli, Wang Lanfen, Wu Jing, Zhu Zhendong
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2021 Jun 17;12:679629. doi: 10.3389/fpls.2021.679629. eCollection 2021.
Fusarium wilt is a destructive soil-borne disease that threatens the production of mung bean. Mung bean lines Zheng8-4 and Zheng8-20 show high resistance and high susceptibility to Fusarium wilt, respectively. Transcriptome analysis was carried out to identify candidate genes involved in Fusarium wilt resistance using Zheng8-4 and Zheng8-20 at 0, 0.5, 1, 2, and 4 days post inoculation (dpi). Differential expression analysis showed that 3,254 genes responded to pathogen infection and were differentially expressed in the resistant and susceptible lines. Weighted gene co-expression network analysis (WGCNA) was also performed to identify five modules highly correlated with Fusarium wilt resistance, in which 453 differentially expressed genes (DEGs) were considered likely to be involved in Fusarium wilt resistance. Among these DEGs, we found 24 genes encoding resistance (R) proteins, 22 encoding protein kinases, 20 belonging to transcription factor families, 34 encoding proteins with oxidoreductase activity, 17 involved in stimulation/stress responses, and 54 annotated to pathogen resistance-related pathways. Finally, 27 annotated genes were further selected as candidate genes of Fusarium wilt resistance in mung bean. This study identifies novel potential resistance-related genes against Fusarium wilt and provides a theoretical basis for further investigation of Fusarium wilt resistance in mung bean breeding.
枯萎病是一种具有破坏性的土传病害，威胁着绿豆的生产。绿豆品种郑8 - 4和郑8 - 20分别对枯萎病表现出高抗性和高感病性。在接种后0、0.5、1、2和4天，利用郑8 - 4和郑8 - 20进行转录组分析，以鉴定参与枯萎病抗性的候选基因。差异表达分析表明，3254个基因对病原菌感染有响应，且在抗性和感病品系中差异表达。还进行了加权基因共表达网络分析（WGCNA），以鉴定与枯萎病抗性高度相关的五个模块，其中453个差异表达基因（DEG）被认为可能参与枯萎病抗性。在这些DEG中，我们发现24个编码抗性（R）蛋白的基因、22个编码蛋白激酶的基因、20个属于转录因子家族的基因、34个编码具有氧化还原酶活性的蛋白的基因、17个参与刺激/应激反应的基因以及54个注释到病原菌抗性相关途径的基因。最后，进一步选择了27个注释基因作为绿豆枯萎病抗性的候选基因。本研究鉴定了与枯萎病抗性相关的新的潜在基因，并为进一步研究绿豆育种中的枯萎病抗性提供了理论基础。
