Department of General Surgery, The First People's Hospital of Yuhang District, Hangzhou, Zhejiang 311100, China.
Comput Math Methods Med. 2021 Jun 14;2021:3334065. doi: 10.1155/2021/3334065. eCollection 2021.
Liver cancer is one of the most aggressive malignant tumors. It is significant to understand the molecular mechanism of liver cancer cells to develop new treatment plans. Studies have identified that FBP1 serves as a cancer inhibitor gene. To research the effect mechanism of FBP1 in liver cancer cells, bioinformatics analysis was performed to study its expression in liver cancer tissue. Survival analysis was also performed. Moreover, starBase database was applied to predict upstream regulatory genes of FBP1. Dual-luciferase assay was performed to testify their targeted relationship. The mRNA and protein expression levels of FBP1 in liver cancer cells were detected by qRT-PCR and western blot, respectively. Cell viability was analyzed by CCK-8 assay. The migratory and invasive abilities of cells were analyzed by Transwell assay. The apoptosis of liver cancer cells was detected by flow cytometry. The results showed that the expression of FBP1 was downregulated in liver cancer tissue and cells. FBP1 low expression was correlated with the poor prognosis of patients. miR-18a-5p could inhibit FBP1 expression. Overexpression of FBP1 could inhibit the progression of liver cancer cells and promote cell apoptosis. Overexpressing miR-18a-5p could promote the progression of liver cancer cells and inhibit cell apoptosis. However, overexpressing FBP1 simultaneously could reverse the effect. miR-18a-5p and FBP1 are expected to be candidates for liver cancer treatment.
肝癌是最具侵袭性的恶性肿瘤之一。了解肝癌细胞的分子机制对于开发新的治疗方案具有重要意义。研究表明，FBP1 作为一种抑癌基因。为了研究 FBP1 在肝癌细胞中的作用机制，通过生物信息学分析研究其在肝癌组织中的表达。同时进行生存分析。此外，应用 starBase 数据库预测 FBP1 的上游调控基因。通过双荧光素酶报告基因实验验证它们的靶向关系。通过 qRT-PCR 和 Western blot 分别检测肝癌细胞中 FBP1 的 mRNA 和蛋白表达水平。通过 CCK-8 assay 分析细胞活力。通过 Transwell assay 分析细胞的迁移和侵袭能力。通过流式细胞术检测肝癌细胞的凋亡。结果表明，FBP1 在肝癌组织和细胞中表达下调。FBP1 低表达与患者预后不良相关。miR-18a-5p 可抑制 FBP1 的表达。过表达 FBP1 可抑制肝癌细胞的进展并促进细胞凋亡。过表达 miR-18a-5p 可促进肝癌细胞的进展并抑制细胞凋亡。然而，同时过表达 FBP1 可逆转这种作用。miR-18a-5p 和 FBP1 有望成为肝癌治疗的候选物。
