用于成像人体皮肤细胞结构的智能手机共聚焦显微镜
Smartphone confocal microscopy for imaging cellular structures in human skin .
作者信息
Freeman Esther E, Semeere Aggrey, Osman Hany, Peterson Gary, Rajadhyaksha Milind, González Salvador, Martin Jeffery N, Anderson R Rox, Tearney Guillermo J, Kang Dongkyun
机构信息
Department of Dermatology, Harvard Medical School and Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA.
Infectious Diseases Institute, Makerere University College of Health Sciences, Mulago Hospital Complex, P.O. Box 22418, Kampala, Uganda.
出版信息
Biomed Opt Express. 2018 Mar 26;9(4):1906-1915. doi: 10.1364/BOE.9.001906. eCollection 2018 Apr 1.
We report development of a low-cost smartphone confocal microscope and its first demonstration of human skin imaging. The smartphone confocal microscope uses a slit aperture and diffraction grating to conduct two-dimensional confocal imaging without using any beam scanning devices. Lateral and axial resolutions of the smartphone confocal microscope were measured as 2 and 5 µm, respectively. confocal images of human skin revealed characteristic cellular structures, including spinous and basal keratinocytes and papillary dermis. Results suggest that the smartphone confocal microscope has a potential to examine cellular details and may help disease diagnosis in resource-poor settings, where conducting standard histopathologic analysis is challenging.
我们报告了一种低成本智能手机共聚焦显微镜的研发及其首次用于人体皮肤成像的演示。该智能手机共聚焦显微镜使用狭缝孔径和衍射光栅来进行二维共聚焦成像，无需使用任何光束扫描设备。该智能手机共聚焦显微镜的横向和轴向分辨率分别测得为2微米和5微米。人体皮肤的共聚焦图像显示出特征性的细胞结构，包括棘层和基底角质形成细胞以及乳头层真皮。结果表明，这种智能手机共聚焦显微镜有潜力检查细胞细节，并可能有助于在资源匮乏地区进行疾病诊断，在这些地区进行标准组织病理学分析具有挑战性。
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