Ibrahim Taghreed A, El Dib Rabab A, Al-Youssef Hanan M, Amina Musarat
Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia / Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia / Department of Pharmacognosy, College of Pharmacy, Helwan University, Helwan, Egypt.
Pak J Pharm Sci. 2019 Jan;32(1):153-163.
It was deemed of interest to investigate Antidesm bunius aerial parts from phytochemical and biological points of view due to limited previous studies. Isolation and identification of phenolic compounds and evaluation of the potential antimicrobial and cytotoxic effects of A. bunius aerial parts was investigated. The petroleum ether (PEE) and 80% EtOH extracts (EE), as well as, n-hexane (HF), CHCl3 (CF), EtOAc (EAF), n-BuOH (BF) and H2O soluble fractions (WF) of the latter were prepared. Phytochemical study has been performed for isolation and identification of the major polyphenols. Antimicrobial activity, using diffusion agar technique, and potential cytotoxic effect against HepG2, MCF7 and HCT cell lines were evaluated. Malic acid (I), caffeic acid (II), methyl benzoate (III), (+)-catechin (IV), (-)-epicatechin (V), epicatechin-(4β→8)-catechin (procynidin B1, VI) and epicatechin-(4β→8)-epicatechin (procyanidin B2, VII) were isolated. Compounds I-VII showed strong to moderate antimicrobial activity, with MIC values in the range of 1.95-125μg/ml except for compounds 1 and IV, which did not show any effect. All tested samples showed dose dependent cytotoxic effect against all three tested cell lines. PEE showed strong cytotoxic activity (IC50=23.7-38.2μg/ml). Furthermore, compound VI showed potent cytotoxicity against HepG2, MCF7 and HCT cell lines (IC=24.7, 16.5 and 18.0μg/ml) respectively. The strong to moderate antimicrobial activity and cytotoxic effect of the plant could be attributed to its content of phenolic acids, flavan-3-ols and/or proanthocyanidins. These findings were confirmed by results obtained for the isolated compounds.
由于先前的研究有限，从植物化学和生物学角度对紫麻属植物的地上部分进行研究显得很有意义。本研究对紫麻属植物地上部分的酚类化合物进行了分离和鉴定，并评估了其潜在的抗菌和细胞毒性作用。制备了石油醚（PEE）和80%乙醇提取物（EE）以及后者的正己烷（HF）、氯仿（CF）、乙酸乙酯（EAF）、正丁醇（BF）和水溶性部分（WF）提取物。开展了植物化学研究以分离和鉴定主要的多酚类物质。采用琼脂扩散法评估了抗菌活性，并评估了对HepG2、MCF7和HCT细胞系的潜在细胞毒性作用。分离出了苹果酸（I）、咖啡酸（II）、苯甲酸甲酯（III）、（+）-儿茶素（IV）、（-）-表儿茶素（V）、表儿茶素-（4β→8）-儿茶素（原花青素B1, VI）和表儿茶素-（4β→8）-表儿茶素（原花青素B2, VII）。化合物I-VII显示出强至中等程度的抗菌活性（除化合物I和IV无作用外）, 其最低抑菌浓度（MIC）值在1.95-125μg/ml范围内）。所有测试样品对所有三种测试细胞系均显示出剂量依赖性细胞毒性作用。PEE显示出较强的细胞毒性活性（IC50 =23.7-38.2μg/ml）。此外，化合物VI对HepG2、MCF7和HCT细胞系分别显示出较强的细胞毒性（IC =24.7、16.5和18.0μg/ml）。该植物的强至中等程度抗菌活性和细胞毒性作用可能归因于其酚酸、黄烷-3-醇和/或原花青素的含量。分离得到的化合物的结果证实了这些发现.
