Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon.
Antimicrobial and Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Messa, Yaoundé, Cameroon.
Biomed Res Int. 2021 Jun 16;2021:1584141. doi: 10.1155/2021/1584141. eCollection 2021.
Microbial infections are increasing worldwide, and the widespread emergence of antibiotic-resistant pathogens poses a severe threat to public health. Medicinal plants are well-known sources of bioactive ingredients. This study was designed to determine the antimicrobial and antioxidant activities of extracts from . The serial exhaustive extraction method using a solvent of increasing polarity from nonpolar (hexane) to polar (water) was designed to prepare crude extracts; liquid-liquid partition was used to fractionate of active extracts. The extracts and fractions were screened for antimicrobial activity on bacteria and yeasts using the microdilution method. The antioxidant activity was done using DPPH and FRAP assays. Out of the sixteen extracts screened, four (Hex, H2O(H), MeOH(EA), and MeOH) exhibited potency with minimal inhibitory concentration (MIC) values ranging from 31.25 to 500 g/mL. Out of the four extracts, two, including MeOH and MeOH(EA), exhibited DPPH radical scavenging activity with the antiradical power of 8.94 × 10 and 47.96 × 10, respectively, and ferric reducing antioxidant power values ranging from 0.34 to 61.53 g equivalent Vit C/g of extract. The phytochemical screening of the promising crude extracts revealed flavonoids, glycosides, phenols, tannins, terpenoids, saponins, and anthraquinones. This study reports the antimicrobial and antioxidant activities of for the first time. The results showed that the serial exhaustive extraction approach used in this study allowed capturing the antimicrobial and antioxidant metabolites beyond the single extraction, indicating the need for a rigorous choice of an appropriate solvent and method for extracting . Further investigation is needed to characterize the active ingredients present in the promising extracts.
微生物感染在全球范围内不断增加，抗生素耐药性病原体的广泛出现对公共卫生构成了严重威胁。药用植物是生物活性成分的知名来源。本研究旨在确定 提取物的抗菌和抗氧化活性。设计了一种使用溶剂从非极性（己烷）到极性（水）的顺序萃取方法来制备粗提取物；使用液-液分配法对活性提取物进行分级。使用微量稀释法筛选提取物和馏分对细菌和酵母的抗菌活性。使用 DPPH 和 FRAP 测定法测定抗氧化活性。在所筛选的十六种提取物中，有四种（Hex、H2O（H）、MeOH（EA）和 MeOH）表现出活性，最小抑菌浓度（MIC）值在 31.25 至 500μg/mL 之间。在这四种提取物中，有两种，包括 MeOH 和 MeOH（EA），表现出 DPPH 自由基清除活性，其抗自由基能力分别为 8.94×10 和 47.96×10，铁还原抗氧化能力值在 0.34 至 61.53μg Vit C/g 提取物之间。有前途的粗提取物的植物化学筛选显示出类黄酮、糖苷、酚类、单宁、萜类、皂苷和蒽醌。本研究首次报道了 的抗菌和抗氧化活性。结果表明，本研究中使用的顺序萃取方法允许捕获抗菌和抗氧化代谢物，超出了单一提取，表明需要严格选择适当的溶剂和方法来提取 。需要进一步研究以表征有前途的提取物中存在的活性成分。
