Machingauta Auxillia, Mukanganyama Stanley
Bio-Molecular Interactions Analyses Group Department of Biotechnology and Biochemistry University of Zimbabwe, Mt Pleasant, P.O. Box 167, Harare, Zimbabwe.
Department of Therapeutics Natural Products Research Unit African Institute of Biomedical Science and Technology Wilkins Hospital, Block C, Corner J. Tongogara and R. Tangwena, Harare, Zimbabwe.
Adv Pharmacol Pharm Sci. 2024 Aug 23;2024:8858665. doi: 10.1155/2024/8858665. eCollection 2024.
was identified by the WHO as a priority pathogen in which the research and development of new antibiotics is urgently needed. Plant phytochemicals have potential as sources of new antimicrobials. The objective of the study was to determine the antibacterial activity of extracts of selected Zimbabwean medicinal plants against and determine their possible mode of action. Extracts were prepared from the leaves of the eight plants including the bark of using solvents of different polarities. Antibacterial activity was evaluated using the microbroth dilution method coupled with the iodonitrotetrazolium colorimetric assay. The effect of the extracts on membrane integrity was determined by quantifying the amount of protein and nucleic acid leaked from the cells after exposure to the extracts. The effects of the extracts on biofilms were investigated. Toxicity studies were carried out using sheep erythrocytes and murine peritoneal cells. Seven out of eight evaluated plant extracts were found to have antibacterial activity. The acetonie (CAA) extract showed the highest inhibitory activity against with a minimal inhibitory concentration of 125 g/mL. The minimum inhibitory concentration (MIC) of the CAA extract caused a protein leakage of 32 g/mL from . The acetonie (CAA), methanolic (CAM), methanolic (CZM), and methanolic (EAM) extracts inhibited biofilm formation. The EAM extract was shown to disrupt mature biofilms. The potent extracts were nontoxic to sheep erythrocytes and mouse peritoneal cells. The activities shown by the extracts indicate that the plants have potential as sources of effective antibacterial and antibiofilm formation agents against .
被世界卫生组织确定为急需研发新型抗生素的重点病原体。植物植物化学物质有作为新型抗菌剂来源的潜力。本研究的目的是确定所选津巴布韦药用植物提取物对[具体细菌名称未给出]的抗菌活性，并确定其可能的作用方式。使用不同极性的溶剂从包括[具体植物名称未给出]树皮在内的八种植物的叶子中制备提取物。采用微量肉汤稀释法结合碘硝基四氮唑比色法评估抗菌活性。通过量化暴露于提取物后细胞中泄漏的蛋白质和核酸量来确定提取物对膜完整性的影响。研究了提取物对生物膜的影响。使用绵羊红细胞和小鼠腹腔细胞进行毒性研究。在所评估的八种植物提取物中，有七种具有抗菌活性。[具体植物名称未给出]丙酮提取物（CAA）对[具体细菌名称未给出]表现出最高的抑制活性，最低抑菌浓度为125μg/mL。CAA提取物的最低抑菌浓度导致[具体细菌名称未给出]中蛋白质泄漏量为32μg/mL。[具体植物名称未给出]丙酮提取物（CAA）、[具体植物名称未给出]甲醇提取物（CAM）、[具体植物名称未给出]甲醇提取物（CZM）和[具体植物名称未给出]甲醇提取物（EAM）抑制[具体细菌名称未给出]生物膜形成。EAM提取物显示可破坏成熟生物膜。强效提取物对绵羊红细胞和小鼠腹腔细胞无毒。提取物所显示的活性表明这些植物有潜力作为针对[具体细菌名称未给出]的有效抗菌和抗生物膜形成剂的来源。
