Jiao Li, Kourkoumpetis Themistoklis, Hutchinson Diane, Ajami Nadim J, Hoffman Kristi, White Donna L, Graham David Y, Hair Clark, Shah Rajesh, Kanwal Fasiha, Jarbrink-Sehgal Maria, Husain Nisreen, Hernaez Ruben, Hou Jason, Cole Rhonda, Velez Maria, Ketwaroo Gyanprakash, Kramer Jennifer, El-Serag Hashem B, Petrosino Joseph F
Section of Gastroenterology and Hepatology, Department of Medicine, Baylor College of Medicine, Houston, TX, 77030, USA.
Center for Innovations in Quality, Effectiveness and Safety (IQuESt), Michael E. DeBakey VA Medical Center, Houston, TX, 77030, USA.
Microb Ecol. 2022 Apr;83(3):811-821. doi: 10.1007/s00248-021-01789-6. Epub 2021 Jul 5.
Limited data exist on the spatial distribution of the colonic bacteria in humans. We collected the colonic biopsies from five segments of 27 polyp-free adults and collected feces from 13 of them. We sequenced the V4 region of the bacterial 16S rRNA gene using the MiSeq platform. The sequencing data were assigned to the amplicon sequence variant (ASV) using SILVA. Biodiversity and the relative abundance of the ASV were compared across the colonic segments and between the rectal and fecal samples. Bacterial functional capacity was assessed using Tax4fun. Each individual had a unique bacterial community composition (Weighted Bray-Curtis P value = 0.001). There were no significant differences in richness, evenness, community composition, and the taxonomic structure across the colon segments in all the samples. Firmicutes (47%), Bacteroidetes (39%), and Proteobacteria (6%) were the major phyla in all segments, followed by Verrucomicrobia, Fusobacteria, Desulfobacterota, and Actinobacteria. There were 15 genera with relative abundance > 1%, including Bacteroides, Faecalibacterium, Escherichia/Shigella, Sutterella, Akkermansia, Parabacteroides, Prevotella, Lachnoclostridium, Alistipes, Fusobacterium, Erysipelatoclostridium, and four Lachnospiraceae family members. Intra-individually, the community compositional dissimilarity was the greatest between the cecum and the rectum. There were significant differences in biodiversity and the taxonomic structure between the rectal and fecal bacteria. The bacterial community composition and structure were homogeneous across the large intestine in adults. The inter-individual variability of the bacteria was greater than inter-segment variability. The rectal and fecal bacteria differed in the community composition and structure.
关于人类结肠细菌的空间分布，现有数据有限。我们从27名无息肉成年人的五个结肠段采集了活检样本，并从其中13人那里收集了粪便。我们使用MiSeq平台对细菌16S rRNA基因的V4区域进行了测序。测序数据使用SILVA被分配到扩增子序列变体（ASV）。比较了各结肠段以及直肠和粪便样本之间的生物多样性和ASV的相对丰度。使用Tax4fun评估细菌功能能力。每个个体都有独特的细菌群落组成（加权布雷-柯蒂斯P值 = 0.001）。所有样本中，各结肠段在丰富度、均匀度、群落组成和分类结构上均无显著差异。厚壁菌门（47%）、拟杆菌门（39%）和变形菌门（6%）是所有段中的主要菌门，其次是疣微菌门、梭杆菌门、脱硫杆菌门和放线菌门。有15个属的相对丰度 > 1%，包括拟杆菌属、粪杆菌属、大肠杆菌/志贺氏菌属、萨特氏菌属、阿克曼氏菌属、副拟杆菌属、普雷沃氏菌属、瘤胃球菌属、艾利斯氏菌属、梭杆菌属、丹毒丝菌属，以及四个毛螺菌科家族成员。个体内部，盲肠和直肠之间的群落组成差异最大。直肠和粪便细菌在生物多样性和分类结构上存在显著差异。成年人整个大肠内的细菌群落组成和结构是均匀的。细菌的个体间变异性大于段间变异性。直肠和粪便细菌在群落组成和结构上存在差异。
