Department of Biology Education, Faculty of Teacher Training and Education, Universitas Siliwangi, Jl. Siliwangi No. 24 Tasikmalaya, 46115, West Java, Indonesia.
Department of Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sleman, Yogyakarta, Indonesia.
Microb Pathog. 2021 Sep;158:105075. doi: 10.1016/j.micpath.2021.105075. Epub 2021 Jul 2.
Gene expression of SAP 4-6 based on the detection of mRNA was observed in Candida albicans isolates from HIV-positive patients with oral candidiasis and commensal from healthy individuals. The species of C. albicans strains were selectively isolated from both sources using CHROMagar Chromogenic Media. The obtained isolates were then induced to express SAP 4-6 using SAP 4-6 gene inducer media. Analysis of gene expression was performed on a molecular basis using the RT-PCR method. Molecular analysis of gene expression showed that the isolates CH3 from HIV-positive patients with oral candidiasis could express SAP 4-6 gene, while commensal isolates from healthy people could not. Based on the results of this study, it could be concluded that, in terms of molecular detection, only isolates from HIV-positive patients (CH3) could express their SAP 4-6 gene.
对来自 HIV 阳性口腔念珠菌病患者和健康个体共生菌的白色念珠菌分离株进行基于 mRNA 检测的 SAP 4-6 基因表达观察。使用 CHROMagar 显色培养基从这两个来源选择性分离白色念珠菌菌株。然后使用 SAP 4-6 基因诱导培养基诱导获得的分离株表达 SAP 4-6。使用 RT-PCR 方法在分子基础上进行基因表达分析。基因表达的分子分析表明，来自 HIV 阳性口腔念珠菌病患者的分离株 CH3 可以表达 SAP 4-6 基因，而来自健康人的共生菌分离株则不能。基于这项研究的结果，可以得出结论，在分子检测方面，只有来自 HIV 阳性患者（CH3）的分离株能够表达其 SAP 4-6 基因。
