Powderly W G, Robinson K, Keath E J
Division of Infectious Diseases, Washington University, St Louis, Missouri.
AIDS. 1992 Jan;6(1):81-4. doi: 10.1097/00002030-199201000-00010.
To evaluate the epidemiology of Candida albicans infection in HIV-infected patients with oral lesions using molecular techniques.
Thirty-nine isolates from HIV-positive patients with oral candidiasis were examined using two DNA probes (a Histoplasma capsulatum ribosomal DNA probe that cross-hybridizes with C. albicans and a C. albicans strain-specific probe derived from repetitive sequence DNA). C. albicans obtained from the oral cavity of patients receiving cytotoxic chemotherapy was used as controls.
Using the H. capsulatum ribosomal DNA probe, isolates were shown to members of many distinct classes of C. albicans. Forty-nine per cent (19 out of 39) of isolates were members of the same class; however, 46% (6 out of 13) of control C. albicans isolates were also members of this class. Further analysis of the class-restricted isolates from the HIV-infected patients using the C. albicans strain-specific probe showed that these could be further separated into distinct strains.
These data indicate that strains of C. albicans that cause oral candidiasis in HIV-positive individuals are not clonally restricted and are similar to those colonizing the oral cavity of other severely immunocompromised hosts. Most patients appear to be infected with unique strains of C. albicans.
运用分子技术评估感染人类免疫缺陷病毒（HIV）且患有口腔病变患者的白色念珠菌感染流行病学情况。
采用两种DNA探针（一种与白色念珠菌交叉杂交的荚膜组织胞浆菌核糖体DNA探针以及一种源自重复序列DNA的白色念珠菌菌株特异性探针）对39株来自HIV阳性口腔念珠菌病患者的分离株进行检测。取自接受细胞毒性化疗患者口腔的白色念珠菌用作对照。
使用荚膜组织胞浆菌核糖体DNA探针检测显示，分离株属于许多不同类别的白色念珠菌。49%（39株中的19株）的分离株属于同一类别；然而，46%（13株对照白色念珠菌分离株中的6株）的对照白色念珠菌分离株也属于该类别。使用白色念珠菌菌株特异性探针对来自HIV感染患者的类别受限分离株进行进一步分析表明，这些分离株可进一步分为不同菌株。
这些数据表明，在HIV阳性个体中引起口腔念珠菌病的白色念珠菌菌株并非克隆受限，且与定植于其他严重免疫受损宿主口腔的菌株相似。大多数患者似乎感染的是白色念珠菌的独特菌株。
