Department of Microbiology, College of Natural Sciences, Pukyong National University, Busan 48513, Republic of Korea.
UCLA Children's Discovery and Innovation Institute, Mattel Children's Hospital UCLA, Department of Pediatrics, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
J Microbiol Biotechnol. 2021 Aug 28;31(8):1079-1087. doi: 10.4014/jmb.2105.05004.
Gentisic acid (GA), a benzoic acid derivative present in various food ingredients, has been shown to have diverse pharmaceutical activities such as anti-carcinogenic, antioxidant, and hepatoprotective effects. In this study, we used a co-culture system to investigate the mechanisms of the anti-inflammatory and anti-adipogenic effects of GA on macrophages and adipocytes, respectively, as well as its effect on obesity-related chronic inflammation. We found that GA effectively suppressed lipopolysaccharide-stimulated inflammatory responses by controlling the production of nitric oxide and pro-inflammatory cytokines and modulating inflammation-related protein pathways. GA treatment also inhibited lipid accumulation in adipocytes by modulating the expression of major adipogenic transcription factors and their upstream protein pathways. Furthermore, in the macrophage-adipocyte co-culture system, GA decreased the production of obesity-related cytokines. These results indicate that GA possesses effective anti-inflammatory and anti-adipogenic activities and may be used in developing treatments for the management of obesity-related chronic inflammatory diseases.
没食子酸（GA）是一种存在于各种食品成分中的苯甲酸衍生物，具有多种药物活性，如抗癌、抗氧化和保肝作用。在这项研究中，我们使用共培养系统分别研究了 GA 对巨噬细胞和脂肪细胞的抗炎和抗脂肪生成作用的机制，以及它对肥胖相关慢性炎症的影响。我们发现，GA 通过控制一氧化氮和促炎细胞因子的产生以及调节炎症相关蛋白途径，有效地抑制了脂多糖刺激的炎症反应。GA 处理还通过调节主要脂肪生成转录因子及其上游蛋白途径来抑制脂肪细胞中的脂质积累。此外，在巨噬细胞-脂肪细胞共培养系统中，GA 减少了与肥胖相关的细胞因子的产生。这些结果表明，GA 具有有效的抗炎和抗脂肪生成活性，可用于开发治疗肥胖相关慢性炎症性疾病的方法。
