Nemati Zargaran Fatemeh, Akya Alisha, Ghadiri Keyghobad, Ranjbarian Parivash, Rostamian Mosayeb
Infectious Diseases Research Center, Health Institute, Kermanshah University of Medical Sciences, Parastar Blvd, 6714415333 Kermanshah, Iran.
Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Int J Pept Res Ther. 2021;27(4):2209-2221. doi: 10.1007/s10989-021-10247-3. Epub 2021 Jun 30.
causes various human infections. Ferric enterobactin protein (FepA) is a conserved protein of with high immunogenicity. In the present study, using comprehensive in silico approaches the T and B cell-specific epitopes of FepA were identified. The T (both class I and class II) and B (both linear and conformational) epitopes of FepA were predicted using prediction tools. The predicted epitopes were screened for human similarity, immunogenicity, antigenicity, allergenicity, toxicity, conservancy, structural and physicochemical suitability, and in case of T epitopes binding to HLA alleles, using numerous immune-informatics, homology modeling, and molecular docking approaches. These analyses led to introduce the most dominant FepA epitopes that are appropriate for vaccine development. Furthermore, we introduced an antigenic peptide containing both T and B epitopes which comprises suitable structural and physiochemical properties needed for vaccine development and it is conserved in many bacteria. Altogether, here the highly immunogenic T and B epitopes of FepA as well as a final epitopic peptide containing both T and B epitopes were found and introduced for future vaccine development studies. It is suggested that the actual efficiency and efficacy of our final epitopic peptide be investigated by in vitro/in vivo testing.
The online version contains supplementary material available at 10.1007/s10989-021-10247-3.
可引起多种人类感染。铁肠杆菌素蛋白（FepA）是一种具有高免疫原性的保守蛋白。在本研究中，使用综合的计算机方法鉴定了FepA的T细胞和B细胞特异性表位。使用预测工具预测了FepA的T（I类和II类）和B（线性和构象）表位。使用众多免疫信息学、同源建模和分子对接方法，对预测的表位进行了人类相似性、免疫原性、抗原性、致敏性、毒性、保守性、结构和物理化学适宜性的筛选，对于T表位，还筛选了其与HLA等位基因的结合情况。这些分析得出了最主要的适合疫苗开发的FepA表位。此外，我们引入了一种包含T和B表位的抗原肽，其具有疫苗开发所需的合适结构和物理化学性质，并且在许多细菌中保守。总之，本文发现并引入了FepA具有高免疫原性的T和B表位以及一种同时包含T和B表位的最终表位肽，用于未来的疫苗开发研究。建议通过体外/体内试验研究我们最终表位肽的实际效率和效力。
在线版本包含可在10.1007/s10989-021-10247-3获取的补充材料。
