Dabbs E R, Sole G J
CSIR Laboratory for Molecular and Cell Biology, Braamfontein, South Africa.
Mol Gen Genet. 1988 Jan;211(1):148-54. doi: 10.1007/BF00338406.
A primarily genetic approach was employed to obtain plasmids in Rhodococcus erythropolis ATCC 12674 which carried genes conferring increased resistance to sodium arsenate and arsenite, cadmium chloride, and chloramphenicol. The plasmids were large, migrating more slowly than chromosomal DNA in agarose gels, and were made up of resistance determinants from the host organism together with part of the genome of nocardiophage Q4. Purified plasmid was used to transform a suitable recipient to increased resistance to sodium arsenate, sodium arsenite, and cadmium chloride.
采用一种主要基于遗传学的方法来获取红平红球菌ATCC 12674中的质粒，这些质粒携带赋予对砷酸钠、亚砷酸盐、氯化镉和氯霉素更高抗性的基因。这些质粒很大，在琼脂糖凝胶中迁移速度比染色体DNA慢，并且由宿主生物体的抗性决定簇以及诺卡氏噬菌体Q4的部分基因组组成。纯化后的质粒用于转化合适的受体，以增强其对砷酸钠、亚砷酸盐和氯化镉的抗性。
