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链霉菌中溴化乙锭诱导的质粒消除及突变产生

Plasmid curing and generation of mutations induced with ethidium bromide in streptomycetes.

作者信息

Crameri R, Davies J E, Hütter R

出版信息

J Gen Microbiol. 1986 Mar;132(3):819-24. doi: 10.1099/00221287-132-3-819.

DOI:10.1099/00221287-132-3-819
PMID:3734751
Abstract

The DNA-intercalating agent ethidium bromide was used to eliminate plasmid DNA from streptomycetes. Other mutational events associated with chromosome changes occurred at high frequency; they resulted in phenotypic changes such as loss of enzyme activity, antibiotic resistance or ability to sporulate.

摘要

DNA嵌入剂溴化乙锭被用于从链霉菌中消除质粒DNA。与染色体变化相关的其他突变事件高频发生;它们导致了诸如酶活性丧失、抗生素抗性或产孢能力等表型变化。

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