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通过结合增强共焦显微镜和量子点三体激子成像来提高图像分辨率。

Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging.

机构信息

Institute for Biophysical Chemistry, OE4350, Hannover Medical School, Fritz-Hartmann-Centre for Medical Research, Germany.

Division for Structural Biochemistry, OE8830, Hannover Medical School, Hannover, Germany.

出版信息

FEBS Open Bio. 2021 Dec;11(12):3324-3330. doi: 10.1002/2211-5463.13246. Epub 2021 Jul 26.

DOI:10.1002/2211-5463.13246
PMID:34228908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8634860/
Abstract

Super-resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3-fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state-of-the-art commercial laser scanning microscope systems.

摘要

超分辨率荧光成像提供了关于亚细胞结构的组成、组织和动态的关键改进信息。量子点三体激子成像 (QDTI) 已被引入为一种易于使用的亚衍射成像方法，当与传统共聚焦显微镜一起使用时，分辨率可提高近 2 倍。在这里，我们通过将 QDTI 与最先进的商业激光扫描显微镜系统相结合，报告了与传统共聚焦显微镜相比，横向和轴向分辨率总体提高了 3 倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/c46a77a8df52/FEB4-11-3324-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/4ed9938578b5/FEB4-11-3324-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/a3038394d74d/FEB4-11-3324-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/c46a77a8df52/FEB4-11-3324-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/4ed9938578b5/FEB4-11-3324-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/a3038394d74d/FEB4-11-3324-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d615/8634860/c46a77a8df52/FEB4-11-3324-g002.jpg

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本文引用的文献

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Nat Methods. 2020 Feb;17(2):217-224. doi: 10.1038/s41592-019-0688-0. Epub 2020 Jan 13.
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Super-resolution microscopy demystified.超分辨率显微镜解析。
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Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.
通过量子点的三光子吸收实现标准共聚焦显微镜下细胞结构的亚衍射分辨率成像的简单方法。
PLoS One. 2013 May 21;8(5):e64023. doi: 10.1371/journal.pone.0064023. Print 2013.
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Quantum dot triexciton imaging with three-dimensional subdiffraction resolution.具有三维亚衍射分辨率的量子点三激子成像
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