Confocal and 2-Photon Microscopy Core Facility, Max-Delbrueck-Center for Molecular Medicine Berlin, Berlin, Germany.
PLoS One. 2013 May 21;8(5):e64023. doi: 10.1371/journal.pone.0064023. Print 2013.
This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.
本研究描述了一种简单的技术，该技术改进了最近开发的基于三光子吸收商用量子点的三维亚衍射成像方法。该方法结合了量子点中三激子产生的生物样品成像与去卷积和光谱复用，为甚至厚的生物样品的多色成像提供了一种新颖的方法，其空间分辨率提高了 1.4 到 1.9 倍。该方法在配备标准连续波激光器的传统共焦显微镜上实现。我们证明了结合去卷积的多色三激子量子点成像在慢病毒转导细胞中的病毒囊泡以及三维鼠源性神经干细胞（神经球）中的中间丝和由分化的 C2C12 成肌细胞堆叠形成的肌管中的密集微管中的应用潜力。
