芦荟多糖通过 Nrf2/HO-1 信号通路和短链脂肪酸代谢改善小鼠急性结肠炎。
Aloe polysaccharides ameliorate acute colitis in mice via Nrf2/HO-1 signaling pathway and short-chain fatty acids metabolism.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, China; School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, China; School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, China.
出版信息
Int J Biol Macromol. 2021 Aug 31;185:804-812. doi: 10.1016/j.ijbiomac.2021.07.007. Epub 2021 Jul 3.
Aloe polysaccharides (APs) are acetyl polysaccharides. It has been reported APs could protect mice from ulcerative colitis (UC), but the complex interactions between APs and the intestinal barrier were unclear. Here, we investigated the relationship between APs and UC, and determined the synergistic effects of Nrf2/HO-1 signaling pathway and short-chain fatty acids (SCFAs) metabolism on protecting intestinal barrier in acute UC mice. Results showed APs could scavenge free radicals in vitro. In vivo, APs had the antioxidant and anti-inflammatory effect both in serum and colon. Besides, the pathological results showed APs could alleviate colonic lesions. Furthermore, our study indicated treatment with APs effectively increased SCFAs production. The inhibition of acute UC in mice was correlated with the APs-mediated effects on improving the expression of ZO-1, occludin, Nrf2, HO-I, and NQO1. Thus, APs effectively promoted the intestinal barrier via Nrf2/HO-1 signaling pathway and SCFAs metabolism, effectively ameliorating acute colitis in mice.
芦荟多糖（APs）是乙酰化多糖。据报道，APs 可以保护小鼠免受溃疡性结肠炎（UC）的侵害，但 APs 与肠道屏障之间的复杂相互作用尚不清楚。在这里，我们研究了 APs 与 UC 之间的关系，并确定了 Nrf2/HO-1 信号通路和短链脂肪酸（SCFAs）代谢对保护急性 UC 小鼠肠道屏障的协同作用。结果表明，APs 可以在体外清除自由基。在体内，APs 在血清和结肠中均具有抗氧化和抗炎作用。此外，病理结果表明 APs 可以减轻结肠损伤。此外，我们的研究表明，APs 的治疗有效地增加了 SCFAs 的产生。APs 对改善 ZO-1、occludin、Nrf2、HO-I 和 NQO1 表达的作用与抑制急性 UC 密切相关。因此，APs 通过 Nrf2/HO-1 信号通路和 SCFAs 代谢有效促进肠道屏障，有效改善了小鼠的急性结肠炎。
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