State Key Laboratory of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province, 214122, China; School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province, 214122, China.
School of Biotechnology, Jiangnan University, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu Province, 214122, China.
Carbohydr Polym. 2021 Aug 1;265:118041. doi: 10.1016/j.carbpol.2021.118041. Epub 2021 Apr 7.
Aloe polysaccharides (APs) are indigestible bioactive polysaccharides, while can be fermented by colonic microbiota. Although plant polysaccharides can alleviate subacute ulcerative colitis (SUC), the mechanisms APs regulated SUC via colonic microbiota have not been fully explored. Hence, to elucidate the complex interactions between the novel APs, colonic microbiota, SCFAs, and inflammation, the SUC mouse model and in-depth analysis were performed, including multiple bioinformatics analysis and structural equation modeling (SEM). After APs intervention, SCFAs and SCFAs-producing genus, including Akkermansia and Blautia, were increased in colon, and the colonic inflammation and barrier dysfunction were alleviated significantly in SUC mice. Spearman analysis found positive correlations between microbiota and SCFAs. PICRUSt2 and KEGG analysis revealed 6-pyruvoyltetra hydropterin synthase in folate biosynthesis metabolism pathway was activated, while phosphotransferase system was inhibited. SEM results further proved APs was beneficial to gut micro-ecological balance in mice via SCFAs metabolism and anti-inflammatory functions. Together, APs could be exploited to alleviate SUC as dietary therapeutics.
芦荟多糖（APs）是一种不可消化的生物活性多糖，但可以被结肠微生物群发酵。虽然植物多糖可以缓解亚急性溃疡性结肠炎（SUC），但 APs 通过结肠微生物群调节 SUC 的机制尚未完全探索。因此，为了阐明新型 APs、结肠微生物群、SCFAs 和炎症之间的复杂相互作用，进行了 SUC 小鼠模型和深入分析，包括多种生物信息学分析和结构方程建模（SEM）。APs 干预后，SCFAs 和产生 SCFAs 的属，包括 Akkermansia 和 Blautia，在结肠中增加，SUC 小鼠的结肠炎症和屏障功能障碍得到显著缓解。Spearman 分析发现微生物群与 SCFAs 之间存在正相关。PICRUSt2 和 KEGG 分析显示，叶酸生物合成代谢途径中的 6- 吡咯并 -5,6- 二氢蝶呤合酶被激活，而磷酸转移酶系统被抑制。SEM 结果进一步证明，APs 通过 SCFAs 代谢和抗炎功能有益于小鼠肠道微生态平衡。总之，APs 可以作为膳食治疗剂来缓解 SUC。
