Department of Oral & Cranio-Maxillofacial Surgery, National Clinical Research Centre for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Clinical and Translational Research Centre for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Biomaterials. 2021 Sep;276:120997. doi: 10.1016/j.biomaterials.2021.120997. Epub 2021 Jun 29.
Implantable self-powered generators (ISPGs) have been extensively explored as energy supplies for driving electronics and electrically stimulated therapeutics in vivo. However, some drawbacks arise, such as complicated architectonics, inescapability of wire connection, energy instability, and consumption. In this study, a host-coupling bio-nanogenerator (HCBG) is developed to configure a self-powered regional electrical environment for powerful bone regeneration. An HCBG consists of a porous electret nanofiber mat coupled with interstitial fluid and stimulated objects of the host after implantation, forming a host coupling effect. This bio-nanogenerator not only overcomes the disadvantages of general ISPGs, but also accomplishes both biomechanical energy scavenging and electrical stimulation therapeutics. The enhancement of osteogenesis differentiation of bone marrow mesenchymal stem cells in vitro and bone regeneration in vivo are remarkably achieved. Moreover, osteogenic ability is systematically evaluated by regulating the electrical performance of HCBGs. Osteogenic differentiation is activated by upregulating more cytosolic calcium ion, following to activate the calcium ion-induced osteogenic signal pathway, while applying electrical stimulation. As an implantable medical technology, the HCBG provides an explorative insight to facilitate the development of ISPG-based electrical medical therapeutics.
植入式自供电发电机（ISPG）已被广泛探索作为驱动电子设备和体内电刺激治疗的能源。然而，一些缺点也随之出现，如结构复杂、无法避免连线、能量不稳定和消耗等问题。在本研究中，我们开发了一种宿主耦合生物纳米发电机（HCBG），以构建一个自供电的局部电环境，用于强大的骨再生。HCBG 由多孔驻极体纳米纤维垫与植入后的宿主间隙液和刺激物耦合而成，形成宿主耦合效应。这种生物纳米发电机不仅克服了一般 ISPG 的缺点，还实现了生物力学能量收集和电刺激治疗。体外骨髓间充质干细胞成骨分化和体内骨再生得到显著增强。此外，通过调节 HCBG 的电性能，系统地评估了其成骨能力。通过上调更多的细胞质钙离子来激活成骨信号通路，从而激活成骨分化，同时施加电刺激。作为一种植入式医疗技术，HCBG 为基于 ISPG 的电医疗治疗的发展提供了探索性的见解。
