Shafaei Hajar, Kalarestaghi Hossein
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Stem Cell Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.
J Cell Physiol. 2020 Nov;235(11):8371-8386. doi: 10.1002/jcp.29681. Epub 2020 Apr 2.
Mesenchymal stem cells (MSCs) are a major component of various forms of tissue engineering. MSCs have self-renewal and multidifferential potential. Osteogenic differentiation of MSCs is an area of attention in bone regeneration. One form of MSCs are adipose-derived stem cells (ASCs), which can be simply harvested and differentiated into several cell lineages, such as chondrocytes, adipocytes, or osteoblasts. Due to special properties, ASCs are frequently used in vitro and in vivo bone regeneration. Identifying factors involved in osteogenic differentiation of ASCs is important for better understanding the mechanism of osteogenic differentiation. Different methods are used to stimulate osteogenesis of ASCs in literature, including common osteogenic media, growth factors, hormones, hypoxia, mechanical and chemical stimuli, genetic modification, and nanotechnology. This review article provides an overview describing the isolation procedure, characterization, properties, current methods for osteogenic differentiation of ASCs, and their basic biological mechanism.
间充质干细胞（MSCs）是各种组织工程形式的主要组成部分。MSCs具有自我更新和多向分化潜能。MSCs的成骨分化是骨再生领域的一个研究热点。脂肪来源干细胞（ASCs）是MSCs的一种形式，它可以简单地获取并分化为多种细胞谱系，如软骨细胞、脂肪细胞或成骨细胞。由于其特殊性质，ASCs常用于体外和体内骨再生。识别参与ASCs成骨分化的因素对于更好地理解成骨分化机制很重要。文献中使用了不同方法来刺激ASCs的成骨作用，包括常见的成骨培养基、生长因子、激素、缺氧、机械和化学刺激、基因修饰以及纳米技术。这篇综述文章概述了ASCs的分离程序、特征、性质、当前的成骨分化方法及其基本生物学机制。
