Ishigure Hiroshi, Kawaki Harumi, Shintani Kohei, Ueno Kyohei, Mizuno-Kamiya Masako, Takayama Eiji, Hotta Masato, Kondoh Nobuo, Nikaido Toru
Department of Operative Dentistry, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry.
Department of Oral Biochemistry, Division of Oral Structure, Function, and Development, Asahi University School of Dentistry.
Dent Mater J. 2021 Dec 1;40(6):1329-1337. doi: 10.4012/dmj.2020-390. Epub 2021 Jul 7.
Numerous studies have shown that the sustained release of ions from dental restorative materials have acid buffering capacity, prevents tooth enamel demineralization, and inhibits bacterial adhesion. Herein, the release behavior and bioresponsiveness of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers were investigated in different types of media based on human dental pulp-derived stem cell (hDPSC) responses. The hDPSCs were cultured for 1-7 days in S-PRG eluates diluted with varying amounts of cell culture media. S-PRG released several types of ions, such as F, Sr, Na, Al, BO, and SiO. The balance of eluted ions differed depending on the dilution and solvent, which in turn affected the cytotoxicity, cell morphology, cell proliferation, and alkane phosphatase activity of hDPSCs, among other properties. The results suggest that tailored S-PRG filler eluates could be designed and prepared for application in dental practice.
大量研究表明，牙科修复材料持续释放的离子具有酸缓冲能力，可防止牙釉质脱矿，并抑制细菌黏附。在此，基于人牙髓干细胞（hDPSC）的反应，研究了表面预反应玻璃离子（S-PRG）填料释放的离子在不同类型介质中的释放行为和生物反应性。将hDPSC在用不同量细胞培养基稀释的S-PRG洗脱液中培养1-7天。S-PRG释放出几种类型的离子，如F、Sr、Na、Al、BO和SiO。洗脱离子的平衡因稀释度和溶剂而异，这反过来又影响了hDPSC的细胞毒性、细胞形态、细胞增殖和碱性磷酸酶活性等特性。结果表明，可以设计和制备定制的S-PRG填料洗脱液用于牙科实践。
