Kaga Masayuki, Kakuda Shinichi, Ida Yusuke, Toshima Hirokazu, Hashimoto Masanori, Endo Kazuhiko, Sano Hidehiko
Division of Biomaterials and Bioengineering Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan.
Eur J Oral Sci. 2014 Feb;122(1):78-83. doi: 10.1111/eos.12107. Epub 2013 Dec 28.
The buffering capacity and inhibitory effects on enamel demineralization of two commercially available dental sealants were evaluated in this study. The effects of filler particles were also examined. Disks of enamel and cured sealant materials of BeautiSealant (silica or S-PRG filler) or Teethmate F-1 were incubated in lactic acid solutions (pH 4.0) for 1-6 d. The pH changes and amounts of ions released in the solutions were assessed, and enamel surfaces were observed using a scanning electron microscope. The pH of the solution with BeautiSealant (S-PRG filler) was neutralized from pH 4.0 to pH 6.1 (after incubation for 1 d) and from pH 4.0 to pH 6.7 (after incubation for 6 d). In addition, no release of calcium ions was detected and the enamel surface was morphologically intact in scanning electron microscopy images. However, the pH of the solution with Teethmate F-1 remained below pH 4.0 during incubation from days 1 to 6. Calcium release was increased in solutions up to and after 6 d of incubation. Scanning electron microscopy images showed that the structures of hydroxyapatite rods were exposed at the specimen surfaces as a result of demineralization. Ions released from S-PRG filler-containing dental sealant rapidly buffered the lactic acid solution and inhibited enamel demineralization.
本研究评估了两种市售牙科密封剂对牙釉质脱矿的缓冲能力和抑制作用。同时也研究了填料颗粒的影响。将牙釉质盘以及BeautiSealant（含二氧化硅或S-PRG填料）或Teethmate F-1的固化密封剂材料在乳酸溶液（pH 4.0）中孵育1至6天。评估溶液中的pH变化和离子释放量，并使用扫描电子显微镜观察牙釉质表面。含BeautiSealant（S-PRG填料）溶液的pH值在孵育1天后从pH 4.0中和至pH 6.1，孵育6天后从pH 4.0中和至pH 6.7。此外，未检测到钙离子释放，扫描电子显微镜图像显示牙釉质表面形态完整。然而，含Teethmate F-1溶液在孵育第1天至第6天期间pH值仍低于pH 4.0。孵育6天及6天后溶液中的钙释放量增加。扫描电子显微镜图像显示，由于脱矿作用，标本表面的羟基磷灰石棒结构暴露出来。含S-PRG填料的牙科密封剂释放的离子迅速缓冲了乳酸溶液并抑制了牙釉质脱矿。
