He G, Tao Q, Liu C, Zhang D, Zhou Y, Liu R
School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515 China.
Nan Fang Yi Ke Da Xue Xue Bao. 2021 Jun 20;41(6):909-915. doi: 10.12122/j.issn.1673-4254.2021.06.14.
To prepare Mn-doped Prussian blue nanoparticles (Mn-PB NPs) for T1-T2 dual-mode magnetic resonance imaging (MRI) and photothermal therapy .
Mn-PB NPs were prepared based on manganese chloride, ferrous chloride and potassium ferricyanide using the microemulsion method. The performance of T1-T2 dual-mode MRI with Mn-PB NPs and the photothermal property of the nanoparticles were assessed. CCK-8 assay and AM/PI double staining were used to evaluate the effect of photothermal therapy using the parepared nanoparticles.
The prepared Mn-PB NPs had a mean particle size of 39.46±0.42 nm with a Zeta potential of -25.9±1.2 mV and exhibited a good dispersibility and uniform particle size. In MRI using the nanoparticles, the r1 and r2 values reached 0.68 and 3.65 (mmol/L)s, respectively, indicating good performance of Mn-PB NPs for T1 and T2 enhancement in MRI. When irradiated with 808 nm laser for 10 min, Mn-PB NPs showed a temperature rise to 90 ℃ to cause significant reduction of cell survival. CCK-8 assay and AM/PI double staining confirmed that Mn-PB NPs were capable of efficient killing of HepG2 cells upon 808 nm laser irradiation.
The Mn-PB NPs prepared in this work have uniform particle size and show good performances both in MRI for T1 and T2 enhancement and in photothermal therapy without obvious cytotoxicity.
制备用于T1-T2双模磁共振成像（MRI）和光热治疗的锰掺杂普鲁士蓝纳米颗粒（Mn-PB NPs）。
基于氯化锰、氯化亚铁和铁氰化钾，采用微乳液法制备Mn-PB NPs。评估了Mn-PB NPs的T1-T2双模MRI性能以及纳米颗粒的光热性能。采用CCK-8法和AM/PI双染法评估所制备纳米颗粒的光热治疗效果。
所制备的Mn-PB NPs平均粒径为39.46±0.42 nm，Zeta电位为-25.9±1.2 mV，具有良好的分散性和均匀的粒径。在使用纳米颗粒的MRI中，r1和r2值分别达到0.68和3.65（mmol/L）s，表明Mn-PB NPs在MRI中对T1和T2增强具有良好性能。用808 nm激光照射10分钟时，Mn-PB NPs温度升高至90℃，导致细胞存活率显著降低。CCK-8法和AM/PI双染法证实，808 nm激光照射下Mn-PB NPs能够有效杀伤HepG2细胞。
本研究制备的Mn-PB NPs粒径均匀，在T1和T2增强的MRI以及光热治疗中均表现出良好性能，且无明显细胞毒性。
