Nano Biomedicine Group, Science Research Center of Medical School, School of Medicine, Shaoxing University, Shaoxing, 312000, China.
Cixi Institute of Biomedical Engineering, CAS Key Laboratory of Magnetic Materials and Devices and Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, P. R. China.
Adv Healthc Mater. 2020 Jan;9(1):e1900948. doi: 10.1002/adhm.201900948. Epub 2019 Nov 20.
Prussian blue nanoprobes are widely studied and applied in tumor photothermal therapy (PTT) and magnetic resonance imaging (MRI), due to their low toxicity and excellent in vivo performance. However, the sizes of hitherto reported Prussian blue nanoprobes are generally larger than 50 nm, which greatly influence cell phagocytosis, in vivo circulation, and biodistribution. In this work, a novel method of doping zinc ions is used to control the size of Prussian blue nanoprobes. Consequently, the performances of the nanoprobes in PTT and MRI are both significantly improved. The results show that the minimum size of Prussian blue nanoprobes achieved by doping 10% zinc ions (abbreviated as SPBZn(10%)) is 3.8 ± 0.90 nm, and the maximum specific absorption coefficient, photothermal conversion efficiency, and longitudinal relaxation rates are 1.78 L g cm , 47.33%, and 18.40 mm s , respectively. In addition, the SPBZn(10%) nanoprobes provide excellent PTT efficacy on 4T1 tumor cells (killing rate: 90.3%) and breast cancer model (tumor inhibition rate: 69.4%). Toxicological experiment results show that the SPBZn(n%) nanoprobes exhibit no obvious in vitro cytotoxicity and they can be used safely in mice at doses below 100 mg kg . Therefore, SPBZn(10%) nanoprobes can potentially be used for effective cancer theranostics.
普鲁士蓝纳米探针由于其低毒性和优异的体内性能，广泛应用于肿瘤光热治疗（PTT）和磁共振成像（MRI）。然而，迄今为止报道的普鲁士蓝纳米探针的尺寸一般大于 50nm，这极大地影响了细胞吞噬、体内循环和生物分布。在这项工作中，采用掺杂锌离子的新方法来控制普鲁士蓝纳米探针的尺寸。因此，纳米探针在 PTT 和 MRI 中的性能都得到了显著提高。结果表明，掺杂 10%锌离子（简称 SPBZn(10%)）的普鲁士蓝纳米探针的最小尺寸为 3.8±0.90nm，最大比吸收系数、光热转换效率和纵向弛豫率分别为 1.78Lgcm-1、47.33%和 18.40mm s-1。此外，SPBZn(10%)纳米探针对 4T1 肿瘤细胞（杀伤率：90.3%）和乳腺癌模型（肿瘤抑制率：69.4%）具有优异的 PTT 疗效。毒理学实验结果表明，SPBZn(n%)纳米探针在体外无明显细胞毒性，在 100mgkg 以下剂量下可安全用于小鼠。因此，SPBZn(10%)纳米探针可用于有效的癌症治疗。
