Department of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, 43210, USA.
The Ohio State University Comprehensive Cancer Center, The Ohio State University, McCampbell Hall South Room 565, 1581 Dodd Drive, Columbus, OH, 43210, USA.
Sci Rep. 2021 Jul 8;11(1):14126. doi: 10.1038/s41598-021-93526-9.
Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. The molecular characteristics of histologically normal appearing tissue adjacent to the tumor (NAT) from PTC patients are not well characterized. The aim of this study was to characterize the global gene expression profile of NAT and compare it with those of normal and tumor thyroid tissues. We performed total RNA sequencing with fresh frozen thyroid tissues from a cohort of three categories of samples including NAT, normal thyroid (N), and PTC tumor (T). Transcriptome analysis shows that NAT presents a unique gene expression profile, which was not associated with sex or the presence of lymphocytic thyroiditis. Among the differentially expressed genes (DEGs) of NAT vs N, 256 coding genes and 5 noncoding genes have been reported as cancer genes involved in cell proliferation, apoptosis, and/or tumorigenesis. Bioinformatics analysis with Ingenuity Pathway Analysis software revealed that "Cancer, Organismal Injury and Abnormalities, Cellular Response to Therapeutics, and Cellular Movement" were major dysregulated pathways in the NAT tissues. This study provides improved insight into the complexity of gene expression changes in the thyroid glands of patients with PTC.
甲状腺乳头状癌 (PTC) 是最常见的甲状腺癌类型。PTC 患者肿瘤旁组织（NAT）的组织学正常外观的分子特征尚未得到很好的描述。本研究旨在描述 NAT 的全基因表达谱，并将其与正常和肿瘤甲状腺组织进行比较。我们使用来自三个样本类别（NAT、正常甲状腺 (N) 和 PTC 肿瘤 (T)）的新鲜冷冻甲状腺组织进行了总 RNA 测序。转录组分析表明，NAT 呈现出独特的基因表达谱，与性别或淋巴细胞性甲状腺炎无关。在 NAT 与 N 之间差异表达的基因 (DEGs) 中，已有 256 个编码基因和 5 个非编码基因被报道为参与细胞增殖、凋亡和/或肿瘤发生的癌症基因。使用 Ingenuity Pathway Analysis 软件进行的生物信息学分析表明，“癌症、机体损伤和异常、细胞对治疗的反应和细胞运动”是 NAT 组织中主要失调的途径。这项研究提供了对 PTC 患者甲状腺中基因表达变化复杂性的深入了解。
