Sindiani Amer Mahmoud, Zayed Faheem, Alshdaifat Eman Hussein, Rawashdeh Hasan M, Al-Woshah Wesam, Zayed Nada
Department of Obstetrics and Gynecology, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Department of Obstetrics and Gynecology, Irbid Speciality Hospital, Irbid, Jordan.
Risk Manag Healthc Policy. 2021 Jul 2;14:2797-2801. doi: 10.2147/RMHP.S306124. eCollection 2021.
To find out whether couples with predominant female offspring have more chances to produce more female embryos during non-medical pre-implantation genetic diagnosis (PGD) for male sex selection.
A total of 125 couples who had three or more female offspring and underwent non-medical PGD for male sex selection between 2015 and 2019 were included. Nuclear DNA was analyzed by fluorescent in situ hybridization (FISH). Two-chromosome (X, Y), 3-chromosome (21, X, Y), and 5-chromosome (13, 18, 21, X, Y) probes were used for FISH. The standard protocol was followed for sperm processing and embryo culture for IVF and PGD.
In 83.2% of the couples, the ratio of female embryos was higher than male embryos. Independent sample -test showed that there is no significant difference between equal and unequal embryonic groups in patients' age, husbands' age, sperm count, sperm motility, total male embryos, total female embryos, normal male embryos, and normal female embryos. For patients with positive pregnancy outcome, 84.6% had unequal embryonic ratio while 15.4% had equal embryonic ratio. Similarly, patients who were treated by short protocol had 85% of unequal embryonic ratio and 15% had equal ratio.
A greater variability in the female to male embryonic ratio is produced in couples having predominantly female offspring and seeking non-medical PGD for male sex selection.
探究在非医学性胚胎植入前遗传学诊断（PGD）进行性别选择时，生育女孩为主的夫妇是否有更多机会产生更多的女性胚胎。
纳入2015年至2019年间共有三个或更多女性后代且接受非医学性PGD进行性别选择的125对夫妇。通过荧光原位杂交（FISH）分析核DNA。使用两染色体（X、Y）、三染色体（21、X、Y）和五染色体（13、18、21、X、Y）探针进行FISH。体外受精（IVF）和PGD的精子处理及胚胎培养遵循标准方案。
83.2%的夫妇中，女性胚胎的比例高于男性胚胎。独立样本t检验显示，在胚胎数量相等和不相等的组中，患者年龄、丈夫年龄、精子数量、精子活力、男性胚胎总数、女性胚胎总数、正常男性胚胎和正常女性胚胎之间无显著差异。对于妊娠结局为阳性的患者，84.6%的胚胎比例不相等，而15.4%的胚胎比例相等。同样，采用短方案治疗的患者中，85%的胚胎比例不相等，15%的胚胎比例相等。
在生育女孩为主且寻求非医学性PGD进行性别选择的夫妇中，女性与男性胚胎比例存在更大的变异性。
