霍乱弧菌神经氨酸酶对各种抗原抗体反应的刺激作用。
Stimulatory effect of Vibrio cholerae neuraminidase on the antibody response to various antigens.
作者信息
Knop J, Sedlacek H H, Seiler F R
出版信息
Immunology. 1978 Feb;34(2):181-7.
neuraminidase (VCN) splits off peripheral sialic acids from cell membranes. Treatment of tumour cells with VCN increases their immunogenicity and it has been suggested that VCN may unmask hidden tumour specific antigens. On the other hand, it has been shown that VCN can affect the cell-cell interactions of immune cells and it seems possible that the enzyme may have a direct effect on the immune response to antigen . The present report describes the effect of VCN on the antibody response of mice to SRBC, to antigens which do not contain peripheral neuraminic acid such as certain bacterial vaccines and rubella virus, and to the soluble antigen BSA. VCN injected i.m. or i.p., but not i.v., together with the antigens increases; (1) the PFC response to SRBC, (2) the antibody response to various bacteria (), (3) the antibody response to rubella virus, and inhibits tolerance induction by aggregate free BSA. The optimal dose required to stimulate the antibody response is between 0.5 and 50 units per animal. Possible mechanisms of the adjuvant activity of VCN are discussed.
神经氨酸酶（VCN）从细胞膜上裂解掉外周唾液酸。用VCN处理肿瘤细胞可增强其免疫原性，有人提出VCN可能会暴露隐藏的肿瘤特异性抗原。另一方面，已表明VCN可影响免疫细胞的细胞间相互作用，并且该酶似乎有可能对抗原的免疫反应产生直接影响。本报告描述了VCN对小鼠针对绵羊红细胞（SRBC）、不含外周神经氨酸的抗原（如某些细菌疫苗和风疹病毒）以及可溶性抗原牛血清白蛋白（BSA）的抗体反应的影响。与抗原一起经肌肉注射或腹腔注射（而非静脉注射）VCN可增强：（1）对SRBC的空斑形成细胞（PFC）反应，（2）对各种细菌的抗体反应，（3）对风疹病毒的抗体反应，并抑制无聚集物的BSA诱导的耐受性。刺激抗体反应所需的最佳剂量为每只动物0.5至50单位。文中讨论了VCN佐剂活性的可能机制。
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