Green T J, Kreier J P
Infect Immun. 1978 Jan;19(1):138-45. doi: 10.1128/iai.19.1.138-145.1978.
This paper reports the results of a study of the nature of the immune response against Plasmodium berghei parasites by inbred rats. A macrophage-cytophilic antibody specific for malarial antigens was identified and characterized. Detection of the antibody on the macrophage surface was accomplished by the parasite adherence tests and by the indirect fluorescent antibody technique. Isolation and purification of the macrophage-cytophilic and opsonic antibodies from hyperimmune rat serum was accomplished by QAE-Sephadez A-50 elution chromatography, and of the macrophage-cytophilic antibody by adsorption with and elution from syngeneic macrophages as well. Characterization of the cytophilic antibody as immunoglobulin G1 was done by immunoelectrophoresis and by Ouchterlony-type double diffusion in gel. Passive protection tests in weanling inbred rats have demonstrated that the opsonizing antibody conferred some protection against P. berghei. The macrophage-cytophilic antibody, on the other hand, was not protective alone but acted synergistically with the opsonizing antibody.
本文报道了近交系大鼠针对伯氏疟原虫寄生虫免疫反应性质的研究结果。鉴定并表征了一种针对疟疾抗原的巨噬细胞嗜亲抗体。通过寄生虫黏附试验和间接荧光抗体技术在巨噬细胞表面检测到该抗体。通过QAE-葡聚糖A-50洗脱色谱法从超免疫大鼠血清中分离纯化巨噬细胞嗜亲抗体和调理素抗体，也通过与同基因巨噬细胞吸附及洗脱来分离巨噬细胞嗜亲抗体。通过免疫电泳和凝胶中的双向免疫扩散法（Ouchterlony型）将嗜亲抗体表征为免疫球蛋白G1。对断奶近交系大鼠进行的被动保护试验表明，调理素抗体对伯氏疟原虫有一定的保护作用。另一方面，巨噬细胞嗜亲抗体单独并无保护作用，但与调理素抗体协同发挥作用。
