Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China; Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.
Cell Rep. 2021 Jul 13;36(2):109391. doi: 10.1016/j.celrep.2021.109391. Epub 2021 Jun 26.
The immunogenicity of the SARS-CoV-2 proteome is largely unknown, especially for non-structural proteins and accessory proteins. In this study, we collect 2,360 COVID-19 sera and 601 control sera. We analyze these sera on a protein microarray with 20 proteins of SARS-CoV-2, building an antibody response landscape for immunoglobulin (Ig)G and IgM. Non-structural proteins and accessory proteins NSP1, NSP7, NSP8, RdRp, ORF3b, and ORF9b elicit prevalent IgG responses. The IgG patterns and dynamics of non-structural/accessory proteins are different from those of the S and N proteins. The IgG responses against these six proteins are associated with disease severity and clinical outcome, and they decline sharply about 20 days after symptom onset. In non-survivors, a sharp decrease of IgG antibodies against S1 and N proteins before death is observed. The global antibody responses to non-structural/accessory proteins revealed here may facilitate a deeper understanding of SARS-CoV-2 immunology.
SARS-CoV-2 蛋白组的免疫原性在很大程度上是未知的，特别是对于非结构蛋白和辅助蛋白。在这项研究中，我们收集了 2360 份 COVID-19 血清和 601 份对照血清。我们在含有 20 种 SARS-CoV-2 蛋白的蛋白质微阵列上分析这些血清，构建针对 IgG 和 IgM 的抗体反应图谱。非结构蛋白和辅助蛋白 NSP1、NSP7、NSP8、RdRp、ORF3b 和 ORF9b 引起普遍的 IgG 反应。非结构/辅助蛋白的 IgG 模式和动态与 S 和 N 蛋白不同。针对这六种蛋白的 IgG 反应与疾病严重程度和临床结局相关，并且在症状出现后约 20 天急剧下降。在非幸存者中，在死亡前观察到针对 S1 和 N 蛋白的 IgG 抗体急剧下降。这里揭示的针对非结构/辅助蛋白的全球抗体反应可能有助于更深入地了解 SARS-CoV-2 免疫学。
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