Williams Daniel M, Hornsby Hailey R, Shehata Ola M, Brown Rebecca, Gallis Marta, Meardon Naomi, Newman Thomas A H, Plowright Megan, Zafred Domen, Shun-Shion Amber S M, Hodder Anthony J, Bliss Deepa, Metcalfe Andrew, Edgar James R, Gordon David E, Sayers Jon R, Nicklin Martin J, Carroll Miles, Collini Paul J, Brown Stephen, de Silva Thushan I, Peden Andrew A
School of Bioscience, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Department of Infection, Immunity and Cardiovascular Diseases, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, UK.
iScience. 2023 Jul 21;26(7):107056. doi: 10.1016/j.isci.2023.107056. Epub 2023 Jun 7.
The prevalence and strength of serological responses mounted toward SARS-CoV-2 proteins other than nucleocapsid (N) and spike (S), which may be of use as additional serological markers, remains underexplored. Using high-content microscopy to assess antibody responses against full-length StrepTagged SARS-CoV-2 proteins, we found that 85% (166/196) of unvaccinated individuals with RT-PCR confirmed SARS-CoV-2 infections and 74% (31/42) of individuals infected after being vaccinated developed detectable IgG against the structural protein M, which is higher than previous estimates. Compared with N antibodies, M IgG displayed a shallower time-dependent decay and greater specificity. Sensitivity for SARS-CoV-2 seroprevalence was enhanced when N and M IgG detection was combined. These findings indicate that screening for M seroconversion may be a good approach for detecting additional vaccine breakthrough infections and highlight the potential to use HCM as a rapidly deployable method to identify the most immunogenic targets of newly emergent pathogens.
针对除核衣壳（N）和刺突（S）蛋白之外的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）蛋白产生的血清学反应的流行率和强度，可能作为额外的血清学标志物，但仍未得到充分研究。我们使用高内涵显微镜评估针对全长链霉亲和素标记的SARS-CoV-2蛋白的抗体反应，发现85%（166/196）经逆转录聚合酶链反应（RT-PCR）确诊感染SARS-CoV-2的未接种疫苗个体，以及74%（31/42）接种疫苗后感染的个体产生了可检测到的针对结构蛋白M的IgG，这高于先前的估计。与N抗体相比，M IgG的时间依赖性衰减较浅且特异性更高。当联合检测N和M IgG时，SARS-CoV-2血清流行率的敏感性得到提高。这些发现表明，筛查M血清转化可能是检测额外疫苗突破性感染的良好方法，并突出了使用高内涵显微镜作为一种可快速部署的方法来识别新出现病原体的最具免疫原性靶点的潜力。
Zotero 插件
