Köhler Ronny, Pohl Christopher, Walschus Uwe, Zippel Roland, Wilhelm Lutz, Hoene Andreas, Patrzyk Maciej, Schlosser Michael
Department of General Surgery, Visceral, Thoracic and Vascular Surgery, University Medical Center Greifswald, Greifswald, Germany.
Department of Surgery, Elbe-Elster Hospital, Herzberg, Germany.
J Biomed Mater Res A. 2022 Jan;110(1):52-63. doi: 10.1002/jbm.a.37265. Epub 2021 Jul 9.
Experimental studies demonstrated antibodies against matrix and coating of polyester-based vascular prostheses. Thus, this study examined associations of these antibodies with serum cytokines (IL-2, IL-4, and IL-10) and local inflammatory reactions. Rats (n = 8/group) intramuscularly received prosthesis segments [PET-C, PET-G, and PET-A groups: polyethylene terephthalate (PET)-based prostheses coated with bovine collagen and gelatin or human serum albumin, respectively; uncoated polytetrafluoroethylene-based (PTFE) prosthesis], with sham-operated controls. Blood was drawn pre-operatively and weekly until day 22. Polymer-specific or coating-specific antibodies and cytokines were detected by enzyme immunoassays, inflammatory reactions were immunohistochemically evaluated on day 23. Polymer-specific antibodies were detected in all PET-groups using uncoated PET as antigenic target, but not for PTFE or controls, coating-specific antibodies only for PET-A. IL-10 was increased in all PET-groups and correlated with polymer-specific antibodies for PET-G and PET-A. IL-2 was increased for PET-A, but overall correlated with PET-specific antibodies. IL-4 remained unchanged in all groups. Intense local inflammatory reactions (ED1 /ED2 macrophages and T lymphocytes) were found within all PET-groups, but only minor for PTFE or controls. In conclusion, PET-specific antibodies were associated with increased IL-10 and along with concurrent coating-specific antibodies also with increased IL-2, indicating a specific T cell response. Thus, matrix and/or coating of polymeric vascular prostheses elicit distinct systemic immune reactions, probably influencing local inflammatory reactions.
实验研究表明存在针对聚酯基血管假体基质和涂层的抗体。因此，本研究检测了这些抗体与血清细胞因子（白细胞介素-2、白细胞介素-4和白细胞介素-10）以及局部炎症反应之间的关联。将大鼠（每组n = 8只）肌肉内植入假体节段[聚对苯二甲酸乙二酯（PET）-C组、PET-G组和PET-A组：分别涂有牛胶原蛋白和明胶或人血清白蛋白的基于聚对苯二甲酸乙二酯（PET）的假体；未涂层的聚四氟乙烯基（PTFE）假体]，并设置假手术对照组。术前及每周采血直至第22天。通过酶免疫测定法检测聚合物特异性或涂层特异性抗体及细胞因子，在第23天通过免疫组织化学评估炎症反应。以未涂层的PET作为抗原靶点，在所有PET组中均检测到聚合物特异性抗体，但在PTFE组或对照组中未检测到；仅在PET-A组检测到涂层特异性抗体。所有PET组中白细胞介素-10均升高，且与PET-G组和PET-A组的聚合物特异性抗体相关。PET-A组中白细胞介素-2升高，但总体上与PET特异性抗体相关。所有组中白细胞介素-4均无变化。在所有PET组中均发现强烈的局部炎症反应（ED1 /ED2巨噬细胞和T淋巴细胞），但在PTFE组或对照组中反应轻微。总之，PET特异性抗体与白细胞介素-10升高相关，同时涂层特异性抗体也与白细胞介素-2升高相关，表明存在特异性T细胞反应。因此，聚合物血管假体的基质和/或涂层引发了不同的全身免疫反应，可能影响局部炎症反应。
