Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka, 19, 20-033, Lublin, Poland.
Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland.
Sci Rep. 2024 Apr 5;14(1):8025. doi: 10.1038/s41598-024-58730-3.
The modification of the surgical polypropylene mesh and the polytetrafluoroethylene vascular prosthesis with cecropin A (small peptide) and puromycin (aminonucleoside) yielded very stable preparations of modified biomaterials. The main emphasis was placed on analyses of their antimicrobial activity and potential immunomodulatory and non-cytotoxic properties towards the CCD841 CoTr model cell line. Cecropin A did not significantly affect the viability or proliferation of the CCD 841 CoTr cells, regardless of its soluble or immobilized form. In contrast, puromycin did not induce a significant decrease in the cell viability or proliferation in the immobilized form but significantly decreased cell viability and proliferation when administered in the soluble form. The covalent immobilization of these two molecules on the surface of biomaterials resulted in stable preparations that were able to inhibit the multiplication of Staphylococcus aureus and S. epidermidis strains. It was also found that the preparations induced the production of cytokines involved in antibacterial protection mechanisms and stimulated the immune response. The key regulator of this activity may be related to TLR4, a receptor recognizing bacterial LPS. In the present study, these factors were produced not only in the conditions of LPS stimulation but also in the absence of LPS, which indicates that cecropin A- and puromycin-modified biomaterials may upregulate pathways leading to humoral antibacterial immune response.
经抗菌肽（cecropin A，小肽）和氨基核苷类抗生素（嘌呤霉素）修饰的手术用聚丙烯网和聚四氟乙烯血管假体可产生非常稳定的修饰生物材料制剂。研究的重点主要是分析它们的抗菌活性以及对 CCD841 CoTr 模型细胞系的潜在免疫调节和非细胞毒性特性。无论呈可溶形式还是固定化形式，cecropin A 均不会显著影响 CCD 841 CoTr 细胞的活力或增殖。相反，嘌呤霉素在固定化形式下不会引起细胞活力或增殖的显著下降，但在可溶形式下给药时会显著降低细胞活力和增殖。这两种分子通过共价键固定在生物材料表面，形成能够抑制金黄色葡萄球菌和表皮葡萄球菌菌株增殖的稳定制剂。还发现这些制剂诱导参与抗菌保护机制的细胞因子的产生，并刺激免疫反应。这种活性的关键调节剂可能与 TLR4 有关，TLR4 是一种识别细菌 LPS 的受体。在本研究中，这些因子不仅在 LPS 刺激条件下产生，而且在没有 LPS 的情况下也产生，这表明cecropin A 和嘌呤霉素修饰的生物材料可能上调导致体液抗菌免疫反应的途径。
