Counteracts Blood-Milk Barrier Disruption and Moderates the Inflammatory Response in -Induced Mastitis.

is a common mastitis-causing pathogen that can disrupt the blood-milk barrier of mammals. Although Zhang (LCZ) can alleviate mice mastitis, whether it has a prophylactic effect on -induced mastitis through intramammary infusion, as well as its underlying mechanism, remains unclear. In this study, -induced injury models of bovine mammary epithelial cells (BMECs) and mice in lactation were used to fill this research gap. tests of BMECs revealed that LCZ significantly inhibited the adhesion ( < 0.01); reduced the cell desmosome damage; increased the expression of the tight junction proteins claudin-1, claudin-4, occludin, and zonula occludens-1 (ZO-1; < 0.01); and decreased the expression of the inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 ( < 0.01), thereby increasing trans-epithelial electric resistance ( < 0.01) and attenuating the lactate dehydrogenase release induced by ( < 0.01). tests indicated that LCZ significantly reduced the injury and histological score of mice mammary tissues in -induced mastitis ( < 0.01) by significantly promoting the expression of the tight junction proteins claudin-3, occludin, and ZO-1 ( < 0.01), which ameliorated blood-milk barrier disruption, and decreasing the expression of the inflammatory cytokines (TNF-α, IL-1β, and IL-6) in mice mammary tissue ( < 0.01). Our study suggested that LCZ counteracted the disrupted blood-milk barrier and moderated the inflammatory response in -induced injury models, indicating that LCZ can ameliorate the injury of mammary tissue in mastitis.