Splichal Igor, Donovan Sharon M, Splichalova Zdislava, Neuzil Bunesova Vera, Vlkova Eva, Jenistova Vera, Killer Jiri, Svejstil Roman, Skrivanova Eva, Splichalova Alla
Laboratory of Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, 549 22 Novy Hradek, Czech Republic.
Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
Microorganisms. 2019 Aug 20;7(8):273. doi: 10.3390/microorganisms7080273.
Non-typhoid are worldwide spread food-borne pathogens that cause diarrhea in humans and animals. Their multi-drug resistances require alternative ways to combat this enteric pathogen. Mono-colonization of a gnotobiotic piglet gastrointestinal tract with commensal lactobacilli and and with probiotic 1917 and their interference with . Typhimurium infection was compared. The impact of bacteria and possible protection against infection with were evaluated by clinical signs, bacterial translocation, intestinal histology, mRNA expression of villin, claudin-1, claudin-2, and occludin in the ileum and colon, and local intestinal and systemic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both lactobacilli colonized the gastrointestinal tract in approximately 100× lower density compare to Nissle and . Typhimurium. Neither nor suppressed the inflammatory reaction caused by the 24 h infection with Typhimurium. In contrast, probiotic Nissle 1917 was able to suppress clinical signs, histopathological changes, the transcriptions of the proteins, and the inductions of the inflammatory cytokines. Future studies are needed to determine whether prebiotic support of the growth of lactobacilli and multistrain lactobacilli inoculum could show higher protective effects.
非伤寒沙门氏菌是全球范围内传播的食源性病原体，可导致人和动物腹泻。它们的多重耐药性需要采用替代方法来对抗这种肠道病原体。对无菌仔猪胃肠道进行共生乳酸杆菌和益生菌1917的单一定殖，并比较它们对鼠伤寒沙门氏菌感染的干扰。通过临床症状、细菌移位、肠道组织学、回肠和结肠中绒毛蛋白、闭合蛋白-1、闭合蛋白-2和闭合蛋白的mRNA表达以及局部肠道和全身炎症细胞因子IL-8、TNF-α和IL-10的水平，评估细菌的影响以及对感染的可能保护作用。与鼠伤寒沙门氏菌相比，两种乳酸杆菌在胃肠道中的定殖密度均低约100倍。两种乳酸杆菌均未抑制由鼠伤寒沙门氏菌24小时感染引起的炎症反应。相比之下，益生菌1917能够抑制临床症状、组织病理学变化、蛋白质转录以及炎症细胞因子的诱导。未来需要进行研究以确定益生元对乳酸杆菌生长的支持以及多菌株乳酸杆菌接种物是否能显示出更高的保护作用。
