Colonization of Germ-Free Piglets with Commensal , , and Probiotic Nissle 1917 and Their Interference with Typhimurium.

Non-typhoid are worldwide spread food-borne pathogens that cause diarrhea in humans and animals. Their multi-drug resistances require alternative ways to combat this enteric pathogen. Mono-colonization of a gnotobiotic piglet gastrointestinal tract with commensal lactobacilli and and with probiotic 1917 and their interference with . Typhimurium infection was compared. The impact of bacteria and possible protection against infection with were evaluated by clinical signs, bacterial translocation, intestinal histology, mRNA expression of villin, claudin-1, claudin-2, and occludin in the ileum and colon, and local intestinal and systemic levels of inflammatory cytokines IL-8, TNF-α, and IL-10. Both lactobacilli colonized the gastrointestinal tract in approximately 100× lower density compare to Nissle and . Typhimurium. Neither nor suppressed the inflammatory reaction caused by the 24 h infection with Typhimurium. In contrast, probiotic Nissle 1917 was able to suppress clinical signs, histopathological changes, the transcriptions of the proteins, and the inductions of the inflammatory cytokines. Future studies are needed to determine whether prebiotic support of the growth of lactobacilli and multistrain lactobacilli inoculum could show higher protective effects.