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通过DPPH、ABTS和FRAP法测定含肉桂醛的β-环糊精包合物的抗氧化活性。

Antioxidant activity of β-cyclodextrin inclusion complexes containing -cinnamaldehyde by DPPH, ABTS and FRAP.

作者信息

Jo Yeon-Ji, Cho Han-Seul, Chun Ji-Yeon

机构信息

Animal Resources Research Center, Konkuk University, Seoul, 05029 Republic of Korea.

Korea Agency of HACCP Accreditation and Services, Yeonje-ri, 28160 South Korea.

出版信息

Food Sci Biotechnol. 2021 Jun 21;30(6):807-814. doi: 10.1007/s10068-021-00914-y. eCollection 2021 Jun.

DOI:10.1007/s10068-021-00914-y
PMID:34249386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8225751/
Abstract

This study was carried out to observed β-cyclodextrin (β-CD) inclusion complexes containing -cinnamaldehyde (CIN) by using DPPH, ABTS and FRAP assay. Antioxidative ability was compared between pure CIN and β-CD-CIN inclusion complexes and particle size, encapsulation efficiency, and temperature-dependent release of inclusion complexes were investigated. High concentration of β-CD (1.8%) as well as guest oil 1:3 molar ratio (β-CD:CIN) influenced on particle size bigger during self-assembly process. And particle sizes were increased as storage period. In the antioxidant capacity results, pure β-CD (1.8%) was antioxidative without CIN especially at FRAP assay. Antioxidant activity dramatically increased after 1:1 molar ratio (1.8% β-CD:CIN), especially at DPPH assay and ABTS assay. In this study, β-CD complexation enhanced CIN solubility and affected increase the antioxidant activity of the CIN. Moreover, we need to consider that molar ratio of between β-CD concentration and CIN is effective manufacturing condition to improve antioxidant activity of β-CD-CIN inclusion complexes.

摘要

本研究旨在通过DPPH、ABTS和FRAP测定法观察含肉桂醛(CIN)的β-环糊精(β-CD)包合物。比较了纯CIN与β-CD-CIN包合物的抗氧化能力,并研究了包合物的粒径、包封率和温度依赖性释放。高浓度的β-CD(1.8%)以及客体油1:3的摩尔比(β-CD:CIN)在自组装过程中影响粒径变大。并且粒径随着储存时间增加。在抗氧化能力结果中,纯β-CD(1.8%)在无CIN时具有抗氧化性,尤其是在FRAP测定中。在1:1摩尔比(1.8%β-CD:CIN)后抗氧化活性显著增加,尤其是在DPPH测定和ABTS测定中。在本研究中,β-CD络合提高了CIN的溶解度,并影响了CIN抗氧化活性的增加。此外,我们需要考虑β-CD浓度与CIN之间的摩尔比是提高β-CD-CIN包合物抗氧化活性的有效制造条件。

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