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采用DPPH自由基法对巴西植物提取物的抗氧化活性进行筛选。

Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method.

作者信息

Mensor L L, Menezes F S, Leitão G G, Reis A S, dos Santos T C, Coube C S, Leitão S G

机构信息

Departamento de Produtos Naturais e Alimentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Centro de Ciências de Saúde, Bl. A, Ilha do Fundão, Rio de Janeiro, 21941-590 RJ, Brazil.

出版信息

Phytother Res. 2001 Mar;15(2):127-30. doi: 10.1002/ptr.687.

Abstract

Brazilian plant extracts belonging to 16 species of 5 different families (71 extracts) were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical. The ability to scavenge DPPH radical was measured in these experiments by the discoloration of the solution. Ginkgo biloba and rutin, commonly used as antioxidants for medical purposes, were used as standards. Based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC(50) values than the other plant extracts. Among the partitions, the more polar ones (ethyl acetate and n-butanol) are those that generally have higher antioxidant activity (AA).

摘要

测试了来自5个不同科16种植物(共71种提取物)的巴西植物提取物对稳定的DPPH(2,2-二苯基-1-苦基肼水合物)自由基的清除能力。在这些实验中,通过溶液的褪色来测定清除DPPH自由基的能力。常用作医学抗氧化剂的银杏叶和芦丁用作标准品。根据我们的结果,可以说一般而言,马鞭草科植物的乙醇提取物比其他植物提取物表现出更低的半数有效浓度(EC50)值。在各萃取部位中,极性较强的部位(乙酸乙酯和正丁醇部位)通常具有较高的抗氧化活性(AA)。

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