Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), Manaus, Brazil.
Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus, Brazil.
Front Cell Infect Microbiol. 2021 Jun 24;11:614985. doi: 10.3389/fcimb.2021.614985. eCollection 2021.
In a infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage. Here, we evaluated the cytoadhesive capacity of gametocytes to bone marrow endothelial cells (HBMEC) and investigated the involvement of some receptors in the cytoadhesion process by using transfected CHO cells (CHO-ICAM1, CHO-CD36 and CHO-VCAM), wild type (CHO-K1) or deficient in heparan and chondroitin sulfate (CHO-745). cytoadhesion assays were performed using a total of 44 isolates enriched in gametocyte stages by Percoll gradient in the different cell lines. The majority of isolates (88.9%) were able to adhere to HBMEC monolayer. ICAM1 seemed to be the sole receptor significantly involved. CD-36 was the receptor with higher adhesion rate, despite no significance was noticed when compared to CHO-745. We demonstrated that gametocyte adheres to bone marrow endothelial cells. Moreover, gametocytes display the ability to adhere to all CHO cells investigated, especially to CHO-ICAM1. These findings bring insights to the comprehension of the role of the bone marrow as a reservoir and the potential impact on parasite transmission to the vector.
在 感染中，骨髓抽吸物中的配子体分布呈比例增加，表明该器官可能是该寄生虫阶段的储存库。在这里，我们评估了配子体对骨髓内皮细胞（HBMEC）的细胞黏附能力，并通过转染 CHO 细胞（CHO-ICAM1、CHO-CD36 和 CHO-VCAM）、野生型（CHO-K1）或缺乏肝素和硫酸软骨素（CHO-745）来研究某些受体在细胞黏附过程中的参与情况。使用总共 44 个通过 Percol 梯度富集配子体阶段的分离株在不同的细胞系中进行细胞黏附测定。大多数分离株（88.9%）能够黏附到 HBMEC 单层上。ICAM1 似乎是唯一显著参与的受体。CD-36 是黏附率最高的受体，尽管与 CHO-745 相比，这并没有显著性差异。我们证明了配子体黏附到骨髓内皮细胞上。此外，配子体表现出黏附所有研究的 CHO 细胞的能力，尤其是 CHO-ICAM1。这些发现为理解骨髓作为储存库的作用以及对寄生虫向媒介传播的潜在影响提供了新的见解。
