Epstein E H, Munderloh N H
J Biol Chem. 1978 Mar 10;253(5):1336-7.
Human skin was sliced with a dermatome, and the ratio of type I to type III collagens at various depths was assayed by comparing the quantities of peptides of each derived from cyanogen bromide digestion of the cut skin. Although immunofluorescent studies have suggested type III collagen is located predominantly beneath the epidermis and around appendages, biochemical determination demonstrates the same ratio of type I to type III collagen at all levels of the dermis even in the absence of cutaneous appendages.
用皮肤切片机切取人类皮肤，通过比较切割皮肤经溴化氰消化后产生的每种胶原蛋白的肽量，测定不同深度处I型和III型胶原蛋白的比例。尽管免疫荧光研究表明III型胶原蛋白主要位于表皮下方和附属器周围，但生化测定显示，即使在没有皮肤附属器的情况下，真皮各层中I型和III型胶原蛋白的比例也是相同的。
