Office of Research Academic and Innovation, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Reproductive Endocrinology and Infertility Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Biomed Res Int. 2021 Jun 28;2021:5519538. doi: 10.1155/2021/5519538. eCollection 2021.
Autophagy is likely altered in patients with endometriosis. Ovarian steroid hormones seem to affect this changing of the autophagic process.
To study the effect of combined oral contraceptive (COC) pills on the expression of autophagic-related gene and in the ectopic and eutopic endometria of patients with endometriosis. . The present quasiexperimental study recruited 36 women (18-45 years old) with endometrioma and nonendometrioma who were scheduled for surgery. Patients with endometrioma were randomly assigned to either a no-treatment group ( = 12) or a COC group ( = 12). The COC group was prescribed a daily oral pill composed of 3 mg drospirenone and 0.03 mg ethinyl estradiol for 6 weeks before surgery. The control group ( = 12) was composed of women without endometrioma. Ectopic endometriotic and endometrium tissues were collected from the no-treatment and COC groups, whereas the only endometrium was collected from the control group. These tissues were used for real-time PCR to measure the expression of the and genes.
The baseline demographic data were not different among the three groups. The gene expression in endometrium tissue in the COC group was significantly less than that in the no-treatment and control groups ( = 0.011 and 0.029, respectively). No significant difference of endometriotic cyst and gene expression was found between COC and no treatment.
Oral COC pills for 6 weeks continuously before surgery decreased the eutopic endometrial expression (mRNA) of the gene compared to those from healthy normal women and nontreated patients with an endometriotic cyst. The change in the expression of autophagy-related genes was more distinct in eutopic than ectopic endometria. This trial is registered with TCTR20170720002. Registered and enrolled the first patient on 20 July 2017.
子宫内膜异位症患者的自噬可能发生改变。卵巢甾体激素似乎会影响自噬过程的这种变化。
研究复方口服避孕药（COC）对子宫内膜异位症患者异位和在位子宫内膜中自噬相关基因和的表达的影响。本准实验研究招募了 36 名（18-45 岁）患有卵巢子宫内膜异位症和非子宫内膜异位症的妇女，她们计划接受手术。将患有卵巢子宫内膜异位症的患者随机分为未治疗组（n=12）或 COC 组（n=12）。COC 组在手术前每天口服一片由 3mg 屈螺酮和 0.03mg 炔雌醇组成的药丸，持续 6 周。对照组（n=12）由无子宫内膜异位症的妇女组成。从未治疗组和 COC 组收集异位子宫内膜异位症和子宫内膜组织，而对照组仅收集子宫内膜组织。这些组织用于实时 PCR 测量和基因的表达。
三组患者的基线人口统计学数据无差异。COC 组子宫内膜组织中基因的表达明显低于未治疗组和对照组（=0.011 和 0.029）。COC 组与未治疗组的卵巢子宫内膜异位症囊和基因表达无显著差异。
手术前连续 6 周口服 COC 药丸可降低与健康正常女性和未治疗的卵巢子宫内膜异位症患者相比，在位子宫内膜中基因的表达（mRNA）。自噬相关基因表达的变化在在位子宫内膜中比在异位子宫内膜中更为明显。本试验于 2017 年 7 月 20 日在 TCTR 注册并登记了第一个患者。
