Marcon Hiam, Griss Luiz G, Molosse Vitor L, Cecere Bruno G O, Alba Davi F, Leal Karoline W, Galli Gabriela M, Souza Carine F, Baldissera Matheus D, Gundel Samanta, de A Bassotto Vitória, Ourique Aline F, Vedovatto Marcelo, Da Silva Aleksandro S
Department of Animal Science, Universidade Do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
Graduate Program in Animal Science, UDESC, Chapecó, SC, Brazil.
Anim Nutr. 2021 Jun;7(2):521-529. doi: 10.1016/j.aninu.2020.06.014. Epub 2021 Mar 17.
Curcumin-containing nanocapsule powder formulations have not been used in ruminant feed to date, despite the fact that curcumin is known to be a functional food additive. The objective of this study was to determine whether ethyl polymethacrylate (Eudragit L-100) nanocapsules loaded with curcumin (N-CU) would improve health and growth of lambs. Thirty-two male Lacaune lambs (body weight [BW] = 16 ± 0.99 kg; 45 d of age) were randomly assigned to 1 of 4 treatments: T0, T1, T2 and T4, representing supplementation of curcumin at 0, 1, 2, and 4 mg/kg concentrate, respectively. The animals in each treatment were allocated in 4 pens of 2 lambs each (8 lambs per treatment). The experiment lasted 17 d, with samples and measurements collected on d 0, 7, 12, and 17. The T2 lambs had greater average daily gain than T0 lambs. Regression analysis showed that the ideal dose of N-CU to enhance weight gain was 1.89 mg/kg concentrate. There were significant interactions ( 0.05) between treatments × time for hematological variables, particularly for increases in erythrocytes (T2) and reductions in counts of leukocytes, neutrophils, and lymphocytes in T1 and T2. There were significant interactions between treatment × time for total protein, globulin, urea, and triglyceride levels. Stimulation of the antioxidant system was also observed. There were increased levels of non-protein thiols (NPSH), as well as increased activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) in the supplemented animals. Levels of reactive oxygen species (ROS) were lower in the serum of supplemented lambs. In general, the 4 mg/kg dose had no positive effects on growth or health. This was an unexpected result, given the known properties of curcumin. Taken together, these findings suggest that addition of low concentrations of nanoencapsulated curcumin (T1 and T2) in lamb feed improves health, minimizing oxidative stress and generates anti-inflammatory effects that may have contributed indirectly to greater weight gain. Nanocapsules potentiate the effects of curcumin and may emerge as a new tool in animal nutrition.
尽管姜黄素是一种功能性食品添加剂，但含姜黄素的纳米胶囊粉末制剂迄今为止尚未用于反刍动物饲料中。本研究的目的是确定负载姜黄素的聚甲基丙烯酸乙酯（Eudragit L-100）纳米胶囊（N-CU）是否能改善羔羊的健康和生长。32只雄性拉库内羔羊（体重[BW]=16±0.99千克；45日龄）被随机分配到4种处理中的1种：T0、T1、T2和T4，分别代表在浓缩物中添加0、1、2和4毫克/千克的姜黄素。每种处理中的动物被分配到4个围栏中，每个围栏有2只羔羊（每种处理8只羔羊）。实验持续17天，在第0、7、12和17天收集样本并进行测量。T2组羔羊的平均日增重高于T0组羔羊。回归分析表明，促进体重增加的N-CU理想剂量为1.89毫克/千克浓缩物。血液学变量在处理×时间之间存在显著交互作用（P<0.05），特别是红细胞增加（T2组）以及T1和T2组白细胞、中性粒细胞和淋巴细胞计数减少。总蛋白、球蛋白、尿素和甘油三酯水平在处理×时间之间存在显著交互作用。还观察到抗氧化系统受到刺激。补充组动物的非蛋白硫醇（NPSH）水平升高，超氧化物歧化酶（SOD）和谷胱甘肽S-转移酶（GST）活性增加。补充组羔羊血清中的活性氧（ROS）水平较低。总体而言，4毫克/千克的剂量对生长或健康没有积极影响。考虑到姜黄素的已知特性，这是一个意外结果。综上所述，这些发现表明，在羔羊饲料中添加低浓度的纳米包封姜黄素（T1和T2）可改善健康状况，将氧化应激降至最低，并产生抗炎作用，这可能间接促进了体重增加。纳米胶囊增强了姜黄素的作用，可能成为动物营养中的一种新工具。
