Department of Veterinary Science of Turin, University of Turin, Turin, Italy.
Nutrigen LG Distribuzione S.r.l, Prato, Italy.
J Vet Pharmacol Ther. 2021 Nov;44(6):910-918. doi: 10.1111/jvp.13000. Epub 2021 Jul 13.
Canine otitis externa is frequently encountered in veterinary practice, caused by primary factors with bacteria and yeast overgrowth acting as secondary and perpetuating factors. The pharmacological support includes anti-inflammatory, antimicrobials, and antimycotic drugs, but therapeutic failure and antimicrobial resistance are leading to alternative strategies based on phytotherapic products. This study aimed to evaluate an essential oil blend (Otogen ) to treat otitis externa in dogs. The experimental design was divided in: (a) an in vitro approach, based on the European Normative UNI EN 1275:2006, to assess the efficacy of the product against the most frequently isolated microorganisms during otitis externa. (b) an in vivo part, 12 owned dogs presenting with acute otitis externa were enrolled. A significant growth reduction (>99.9%) of Malassezia pachydermatis and Candida albicans after 15 min of contact and Pseudomonas aeruginosa after 1 h of incubation was recorded. For Staphylococcus pseudintermedius, 50% of growth reduction were appreciated after 15 min. Results obtained in vivo after 7 days of blend administration, noted a significant improvement of all the considered parameters (most important were head shaking, erythema, and scraping). The results obtained may support the usefulness of the tested phytotherapic blend to manage acute otitis externa in dogs.
犬外耳炎在兽医临床中较为常见，主要由原发性因素引起，细菌和酵母过度生长则作为继发性和持续性因素。药理支持包括抗炎、抗菌和抗真菌药物，但治疗失败和抗菌药物耐药性导致了基于植物疗法产品的替代策略。本研究旨在评估一种精油混合物（Otogen）治疗犬外耳炎的效果。实验设计分为：（a）体外方法，基于欧洲规范 UNI EN 1275：2006，评估产品对耳炎中最常分离的微生物的疗效。（b）体内部分，纳入 12 只患有急性外耳炎的自有犬。记录到在接触 15 分钟后，马拉色菌和白色念珠菌的生长减少>99.9%，铜绿假单胞菌在孵育 1 小时后减少。对于中间葡萄球菌，在 15 分钟后观察到 50%的生长减少。在混合剂给药 7 天后获得的体内结果表明，所有考虑的参数均显著改善（最重要的是摇头、红斑和刮擦）。所得结果可能支持所测试的植物疗法混合物在外耳炎的管理中具有有用性。
