羟基酪醇、羟基酪醇乙酸酯和硝基羟基酪醇在帕金森病大鼠 MPP 模型中的抗氧化作用。
Antioxidant Effect of Hydroxytyrosol, Hydroxytyrosol Acetate and Nitrohydroxytyrosol in a Rat MPP Model of Parkinson's Disease.
机构信息
Laboratorio de Neuroprotección, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa, CP: 62209, Cuernavaca, Morelos, Mexico.
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, M.V.S., Ciudad de México, Mexico.
出版信息
Neurochem Res. 2021 Nov;46(11):2923-2935. doi: 10.1007/s11064-021-03379-x. Epub 2021 Jul 14.
3,4-Dihydroxyphenyl ethanol, known as hydroxytyrosol (HTy), is a phenylpropanoid found in diverse vegetable species. Several studies have demonstrated that HTy is a potent antioxidant. Thus, our study is aimed to evaluate the antioxidant effect of HTy and its derivatives, hydroxytyrosol acetate (HTyA) and nitrohydroxytyrosol (HTyN), in a model of oxidative stress induced by 1-methyl-4-phenylpyridinium (MPP) in rats. Rats were administered intravenously (i.v.) in the tail with 1 mL saline solution or polyphenol compound (1.5 mg/kg) 5 min before intrastriatal infusion of 10 µg MPP/8 µL. We found that rats injured with MPP, pretreatment with HTy, HTyA or HTyN significantly decreased ipsilateral turns. This result was consistent with a significant preservation of striatal dopamine levels and decreased lipid fluorescence products (LFP), a marker of oxidative stress. Brain GSH/GSSG ratio, from rats pretreated with HTy or HTyN showed a significant preservation of that marker, decreased as a consequence of MPP-induced oxidative damage. These results show an antioxidant effect of HTy, HTyA and HTyN in the MPP model of Parkinson's disease in the rat.
3,4-二羟苯乙醇，又名羟基酪醇（HTy），是一种存在于多种蔬菜物种中的苯丙素。多项研究表明，HTy 是一种有效的抗氧化剂。因此，我们的研究旨在评估 HTy 及其衍生物，即羟基酪醇乙酸酯（HTyA）和硝基羟基酪醇（HTyN）在 1-甲基-4-苯基吡啶鎓（MPP）诱导的大鼠氧化应激模型中的抗氧化作用。大鼠尾静脉注射 1 mL 生理盐水或多酚化合物（1.5 mg/kg），5 分钟后纹状体内注射 10 µg MPP/8 µL。我们发现，用 MPP 损伤的大鼠，用 HTy、HTyA 或 HTyN 预处理后，同侧转弯明显减少。这一结果与纹状体多巴胺水平的显著保存一致，并且降低了脂质荧光产物（LFP），这是氧化应激的一个标志物。来自用 HTy 或 HTyN 预处理的大鼠的脑 GSH/GSSG 比值显示出该标志物的显著保存，由于 MPP 诱导的氧化损伤而降低。这些结果表明 HTy、HTyA 和 HTyN 在 MPP 诱导的大鼠帕金森病模型中具有抗氧化作用。
Zotero 插件