用4-乙酰氨基-4'-异硫氰酸基芪-2,2'-二磺酸（SITS）进行免疫荧光研究。

Immunofluorescence studies with 4-acetamido-4'-isothiocyanato stilbene-2,2'-disulphonic acid (SITS).

作者信息

Rothbarth P H, Tanke H J, Mul N A, Ploem J S, Vliegenthart J F, Ballieux R E

出版信息

J Immunol Methods. 1978;19(2-3):101-9. doi: 10.1016/0022-1759(78)90170-9.

Abstract

The application of the fluorochrome 4-acetamido-4'-isothiocyanato stilbene-2,2'-disulphonic acid (SITS) in immunofluorescence was studied. The optimal excitation wave length is 350 nm, and optimal fluorescence is obtained at 420 nm. After purification of the commercial compound, conjugation is performed in a strong buffer at pH 9.0-9.5. SITS conjugates were very satisfactory for immunofluorescence studies of the cytoplasmic antigens of cell preparations, but their blue emission was difficult to distinguish from autofluorescence in sections of human tissues. Good results with immunofluorescence on membrane bound antigens were obtained by using an ultra-violet laser beam as light source. SITS can be used simultaneously with FITC and TRITC conjugates thus making it possible to show three antigens in one preparation.

摘要

研究了荧光染料4-乙酰氨基-4'-异硫氰酸基芪-2,2'-二磺酸（SITS）在免疫荧光中的应用。最佳激发波长为350nm，在420nm处可获得最佳荧光。纯化市售化合物后，在pH 9.0 - 9.5的强缓冲液中进行偶联。SITS偶联物对于细胞制剂细胞质抗原的免疫荧光研究非常令人满意，但在人体组织切片中，其蓝色发射难以与自发荧光区分开来。通过使用紫外激光束作为光源，在膜结合抗原的免疫荧光方面取得了良好结果。SITS可与FITC和TRITC偶联物同时使用，从而有可能在一份制剂中显示三种抗原。