Department of Biochemistry and Molecular Biology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan.
Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan.
Nucleosides Nucleotides Nucleic Acids. 2021;40(8):790-797. doi: 10.1080/15257770.2021.1950759. Epub 2021 Jul 15.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a pandemic of the coronavirus disease in 2019. RNA-dependent RNA polymerase (RdRp) plays an essential role in RNA replication and transcription in SARS-CoV-2. In this study, we focused on the RNA template component of viral RdRp structure and analyzed human microRNAs (miRNAs) targeting specific sequences in this RNA. By examining miRNA databases and using an RNA-RNA interaction assay, we observed hsa-miR-15b-5p interacting with the RNA component of viral RdRp. Our findings provide evidence that hsa-miR15b-5p may suppresses viral infection and proliferation by targeting the RNA template component of SARS-CoV-2 RdRp.
严重急性呼吸综合征冠状病毒 2 （SARS-CoV-2）于 2019 年引发了冠状病毒病大流行。RNA 依赖性 RNA 聚合酶（RdRp）在 SARS-CoV-2 的 RNA 复制和转录中发挥着重要作用。在本研究中，我们专注于病毒 RdRp 结构中的 RNA 模板组件，并分析了靶向该 RNA 特定序列的人类 microRNAs（miRNAs）。通过检查 miRNA 数据库并使用 RNA-RNA 相互作用测定，我们观察到 hsa-miR-15b-5p 与病毒 RdRp 的 RNA 成分相互作用。我们的研究结果表明，hsa-miR15b-5p 可能通过靶向 SARS-CoV-2 RdRp 的 RNA 模板组件来抑制病毒感染和增殖。
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