State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology (Ministry of Education), School of Life Sciences, Fudan University, Shanghai 200438, China.
Advanced Institute of Natural Sciences, Beijing Normal University at Zhuhai, Zhuhai 440400, China.
Proc Natl Acad Sci U S A. 2021 Jul 20;118(29). doi: 10.1073/pnas.2018799118.
Plant architecture is an important agronomic trait that affects crop yield. Here, we report that a gene involved in programmed cell death, , negatively regulates plant architecture and grain yield in rice. We used the CRISPR/Cas9 system to introduce loss-of-function mutations into in 11 rice cultivars. Targeted mutagenesis of enhanced grain yield and improved plant architecture by increasing plant height and optimizing panicle type and grain shape. Transcriptome analysis showed that knockout affected auxin biosynthesis, as well as the gibberellin and cytokinin biosynthesis and signaling pathways. OsPDCD5 interacted directly with OsAGAP, and positively regulated plant architecture and grain yield in rice. Collectively, these findings demonstrate that is a promising candidate gene for breeding super rice cultivars with increased yield potential and superior quality.
植物结构是一个重要的农艺性状，影响作物产量。在这里，我们报道一个参与细胞程序性死亡的基因， ，负调控水稻的植物结构和籽粒产量。我们使用 CRISPR/Cas9 系统将功能丧失突变引入 11 个水稻品种中。 的靶向诱变增强了籽粒产量，并通过增加株高和优化穗型和粒形来改善植物结构。转录组分析表明， 敲除影响生长素的生物合成，以及赤霉素和细胞分裂素的生物合成和信号通路。OsPDCD5 与 OsAGAP 直接相互作用， 正向调控水稻的植物结构和籽粒产量。总的来说，这些发现表明 是一个很有前途的候选基因，可用于培育具有更高产量潜力和更好品质的超级水稻品种。
