Department of Respiratory Medicine, PLA General Hospital of Southern Theatre Command, Guangzhou, Guangdong, China.
Department of Geriatric Respiratory Medicine, PLA General Hospital of Southern Theatre Command, Guangzhou, Guangdong, China.
Lab Med. 2022 Jan 6;53(1):39-46. doi: 10.1093/labmed/lmab041.
The emerging role of long noncoding RNAs (lncRNAs) in cancer, especially in lung adenocarcinoma (LUAD), is attracting increasingly more attention as a potential therapeutic target. However, whether lncRNA LINC00205 regulates the malignancy of LUAD has not been characterized. In this study, we discovered that LINC00205 was markedly upregulated in LUAD tissues and cell lines and correlated with poor prognosis of patients with LUAD. Our data showed that LINC00205 promoted the migration and proliferation of LUAD cells in vitro and tumor growth in vivo. Notably, the tumor suppressor miR-185-5p was found to be a direct target of LINC00205. In addition, miR-185-5p diminished the promotion of cell proliferation and migration mediated by LINC00205, whereas miR-185-5p inhibition had the opposite effect. In summary, our results show that LINC00205 contributes to LUAD malignancy by sponging miR-185-5p, which provides new insight into LUAD progression.
长链非编码 RNA（lncRNA）在癌症中的作用，尤其是在肺腺癌（LUAD）中的作用，作为一个潜在的治疗靶点，正引起越来越多的关注。然而，lncRNA LINC00205 是否调节 LUAD 的恶性程度尚未得到明确表征。在本研究中，我们发现 LINC00205 在 LUAD 组织和细胞系中明显上调，并与 LUAD 患者的不良预后相关。我们的数据表明，LINC00205 促进了 LUAD 细胞的体外迁移和增殖以及体内肿瘤生长。值得注意的是，肿瘤抑制 miR-185-5p 被发现是 LINC00205 的直接靶标。此外，miR-185-5p 减弱了 LINC00205 介导的细胞增殖和迁移的促进作用，而 miR-185-5p 的抑制则产生相反的效果。总之，我们的研究结果表明，LINC00205 通过海绵吸附 miR-185-5p 促进 LUAD 恶性程度，为 LUAD 进展提供了新的见解。
