Zhong Lingling, Ju KeJu, Chen Ainian, Cao Hua
Department of Neurology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, China.
Front Aging Neurosci. 2021 Jul 1;13:684289. doi: 10.3389/fnagi.2021.684289. eCollection 2021.
Parkinson's disease (PD) is a chronic and progressive degenerative disease of the central nervous system. Degenerative neuropathy can occur in patients with PD even before typical clinical symptoms appear in the preclinical stage. Therefore, if the early diagnosis of degenerative diseases can be timely and the correlation with the disease progression can be explored, the disease progression will be slowed down and the quality of life of patients will be improved. In this study, the circRNA microarray was employed to screen the dysregulated circRNA in plasma samples of PD. Four circRNAs (circ_0085869, circ_0004381, circ_0017204, and circ_0090668) were obtained with increased levels in PD patients by cross comparison and preliminary verification in PD comparing with healthy controls. Further validation found the circRNA panel was consistent with the training set. The ROC curve also revealed a high diagnostic ability of circ_0004381 and circ_0017204 in predicting the early stage of PD from healthy controls. circ_0085869, circ_0004381, circ_0017204, and circ_0090668 also presented a high ability to distinguish the late stage of PD from early stage. In conclusion, circulating circRNA panel might be a potential fingerprint for predicting the early diagnosis of PD and may act as a biomarker for disease progression.
帕金森病（PD）是一种慢性进行性中枢神经系统退行性疾病。在临床前期，甚至在典型临床症状出现之前，PD患者就可能发生退行性神经病变。因此，如果能及时对退行性疾病进行早期诊断，并探索其与疾病进展的相关性，就能减缓疾病进展，提高患者生活质量。在本研究中，采用环状RNA微阵列筛选PD患者血浆样本中表达失调的环状RNA。通过与健康对照进行交叉比较和初步验证，在PD患者中获得了4种表达水平升高的环状RNA（circ_0085869、circ_0004381、circ_0017204和circ_0090668）。进一步验证发现，环状RNA组合与训练集一致。ROC曲线还显示，circ_0004381和circ_0017204在区分健康对照与PD早期方面具有较高的诊断能力。circ_0085869、circ_0004381、circ_0017204和circ_0090668在区分PD晚期与早期方面也具有较高的能力。总之，循环环状RNA组合可能是预测PD早期诊断的潜在标志物，也可能作为疾病进展的生物标志物。
