采用高效液相色谱法对健康居民血清类胡萝卜素浓度进行定量分析。

Quantitation of serum carotenoid concentrations in healthy inhabitants by high-performance liquid chromatography.

作者信息

Ito Y, Sasaki R, Minohara M, Otani M, Aoki K

机构信息

Department of Hygiene, Fujita-Gakuen Health University School of Medicine, Toyoake City, Japan.

出版信息

Clin Chim Acta. 1987 Nov 16;169(2-3):197-207. doi: 10.1016/0009-8981(87)90320-2.

DOI:10.1016/0009-8981(87)90320-2

PMID:3427779

Abstract

Serum carotenoid concentrations in healthy subjects by age and sex were investigated, as determined by two high-performance liquid chromatography (HPLC) methods. Serum 'carotene' concentrations were 0.655 +/- 0.499 mumol/l 35.2 +/- 26.8 micrograms/dl) for 227 males, and 1,395 +/- 0.780 mumol/l (74.9 +/- 41.9 micrograms/dl) for 370 females. In addition, serum concentrations of alpha-carotene, beta-carotene, and lycopene were obtained from another sample included 356 males and 567 females living in the same area. alpha-Carotene for 356 males was 0.085 +/- 0.096 mumol/l (4.6 +/- 5.2 micrograms/dl), beta-carotene was 0.555 +/- 0.491 mumol/l (29.8 +/- 26.4 micrograms/dl), and lycopene was 0.372 +/- 0.273 mumol/l (20.0 +/- 14.7 micrograms/dl), respectively. Serum concentrations of alpha-carotene, beta-carotene as well as concentrations of lycopene in these inhabitants were significantly higher for females than for males (p less than 0.001).

摘要

采用两种高效液相色谱（HPLC）方法测定了健康受试者血清类胡萝卜素浓度，并按年龄和性别进行了调查。227名男性的血清“胡萝卜素”浓度为0.655±0.499μmol/L（35.2±26.8μg/dl），370名女性的血清“胡萝卜素”浓度为1.395±0.780μmol/L（74.9±41.9μg/dl）。此外，从居住在同一地区的356名男性和567名女性的另一份样本中获得了α-胡萝卜素、β-胡萝卜素和番茄红素的血清浓度。356名男性的α-胡萝卜素为0.085±0.096μmol/L（4.6±5.2μg/dl），β-胡萝卜素为0.555±0.491μmol/L（29.8±26.4μg/dl），番茄红素为0.372±0.273μmol/L（20.0±14.7μg/dl）。这些居民中，女性的α-胡萝卜素、β-胡萝卜素血清浓度以及番茄红素浓度均显著高于男性（p＜0.001）。