Katrangi N, Kaplan L A, Stein E A
J Lipid Res. 1984 Apr;25(4):400-6.
We describe a specific assay for serum provitamin A (alpha- and beta-carotene) by high pressure liquid chromatography (HPLC). The system separates alpha- and beta-carotene in 7.4 min using a C18 muBondapak, 10-micron particle size column with a mobile phase of acetonitrile-chloroform 92:8 at 2 ml per min and a 462 nm detector. The HPLC assay had a recovery of 94.8% of added beta-carotene and, at a serum concentration of 215.2 micrograms/L, had within-run and between-run precisions of 3.1% and 3.6%, respectively. In 65 subjects, the HPLC-determined provitamin A (alpha- and beta-carotene) value was 343 +/- 166 micrograms/L and averaged 23.4 +/- 7.9% (range 9-43%) of the values obtained by a traditional colorimetric assay for total serum "carotenes." Although total serum carotenes showed no relationship to serum vitamin A (r = -0.048; P = 0.78), HPLC-determined alpha- and beta-carotene was significantly inversely correlated (r = -0.357; P = 0.05).
我们描述了一种通过高压液相色谱法（HPLC）测定血清中维生素A原（α-和β-胡萝卜素）的特定方法。该系统使用C18 μBondapak（粒径10微米）柱，以乙腈-氯仿（92:8）为流动相，流速为每分钟2毫升，配备462纳米检测器，可在7.4分钟内分离α-和β-胡萝卜素。HPLC法对添加的β-胡萝卜素的回收率为94.8%，在血清浓度为215.2微克/升时，批内精密度和批间精密度分别为3.1%和3.6%。在65名受试者中，HPLC测定的维生素A原（α-和β-胡萝卜素）值为343±166微克/升，平均为传统比色法测定的总血清“胡萝卜素”值的23.4±7.9%（范围9 - 43%）。虽然总血清胡萝卜素与血清维生素A无相关性（r = -0.048；P = 0.78），但HPLC测定的α-和β-胡萝卜素与血清维生素A呈显著负相关（r = -0.357；P = 0.05）。
