Ciurli Alessio, Liebl Maximiliam, Derks Rico J E, Neefjes Jacques J C, Giera Martin
Oncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
iScience. 2021 Jun 24;24(7):102768. doi: 10.1016/j.isci.2021.102768. eCollection 2021 Jul 23.
Saliva is a complex bodily fluid composed of metabolites secreted by major and minor glands, as well as by-products of host oral cells, oral bacteria, gingival crevicular fluid, and exogenous compounds. Major salivary glands include the paired parotid, submandibular, and sublingual glands. The secreted fluids of the salivary glands vary in composition, flow rate, site of release, and clearance suggesting that different types of saliva fulfill different functions and therefore can provide unique biological information. Consequently, for the comprehension of the functionality of the salivary components, spatially resolved investigations are warranted. To understand and comprehensively map the highly heterogeneous environment of the oral cavity, advanced spatial sampling techniques for metabolomics analysis are needed. Here, we present a systematic evaluation of collection devices for spatially resolved sampling aimed at untargeted metabolomics and propose a comprehensive and reproducible collection and analysis protocol for the spatially resolved analysis of the human oral metabolome.
唾液是一种复杂的体液，由大、小唾液腺分泌的代谢产物以及宿主口腔细胞、口腔细菌、龈沟液和外源性化合物的副产品组成。主要唾液腺包括成对的腮腺、颌下腺和舌下腺。唾液腺分泌的液体在成分、流速、释放部位和清除方面各不相同，这表明不同类型的唾液具有不同的功能，因此可以提供独特的生物学信息。因此，为了理解唾液成分的功能，有必要进行空间分辨研究。为了理解和全面绘制口腔高度异质的环境，需要先进的代谢组学分析空间采样技术。在此，我们对用于非靶向代谢组学的空间分辨采样的收集装置进行了系统评估，并提出了一种全面且可重复的收集和分析方案，用于人类口腔代谢组的空间分辨分析。
